提出了高效液相色谱法测定柑橘皮中柠檬苦素含量的方法。样品用体积分数为70%乙醇溶液于50 ℃超声提取75 min。以Sinochrom ODS-BP(250 mm×4.6 mm,10 μm)为分离柱,以乙腈-水(45+55)溶液作为流动相,用紫外检测器在波长210 nm处进行测定。柠檬苦素的质量浓度在12.0~384 mg·L-1范围内与其峰面积呈线性关系,方法的平均回收率为99.9%,相对标准偏差(n=5)为1.8%。

HPLC was applied to the determination of limonin in citrus peels. The sample was ultrasonically extracted with (φ) 70% ethanol solution at 50 ℃ for 75 min. Sinochrom ODS-BP (250 mm×4.6 mm, 10 μm) column was used as stationary phase, a mixture of acetonitrile and water mixed in the ratio of 45 to 55 (by volume) was used as mobile phase; UV-detection at 210 nm was used in the determination. Linear relationship between values of peak area and mass concentration of limonin was obtained in the range of 12.0-384 mg·L-1. Values of average recovery and RSD (n=5) found were 99.9% and 1.8% respectively.