六种氨基醇药物在1-萘万古霉素手性固定相上的对映体分离
Separation of Enantiomers of 6 Alkamine Drugs on 1-Napthalene Vancomycin Chiral Stationary Phase
摘 要
在1-萘万古霉素手性固定相上分离了6种氨基醇类药物的对映体。考察了流动相中的酸、碱添加剂种类和浓度、溶质的化学结构对对映体分离的影响,同时探讨了手性识别机理。结果表明:普萘洛尔、比索洛尔、阿替洛尔、美托洛尔、索他洛尔和沙丁胺醇等6种药物的对映体在1-萘万古霉素手性柱上得到了完全分离,当甲醇流动相中冰乙酸和三乙胺添加剂量均为0.001%(体积分数)时,6种氨基醇的选择因子依次是1.16,1.36,1.15,1.10,1.18,1.16。
1-萘万古霉素
对映体
氨基醇类药物
Chiral stationary phase
1-Napthalene vancomycin
Enantiomers
Alkamine drugs
Abstract
Enantiomers of 6 alkamine drugs, including propranolol, bisoprolol, atenolol, metoprolol, soatlol and salbutamol, were separated on the self-made 1-napthalene vancomycin chiral stationary phase. Influential factors to the separation, including kinds and concentration of acid and alkali as additive, and chemical structure of solute, were studied, and the mechanism of recognition of chirality was discussed. It was found that enantiomers of 6 alkamine drugs were completely separated on the 1-napthalene vancomycin chiral stationary phase. The separation factors were attained to 1.16, 1.36, 1.15, 1.10, 1.18 and 1.16 for the 6 alkamine drugs respectively, when the amounts of the additives glacial acetic acid and triethylamine in the mobile phase were same as (φ %) 0.001%.
中图分类号 O652.63
所属栏目 试验与研究
基金项目 国家自然科学基金(No.81102408);昆明医学院2011年研究生创新基金
收稿日期 2011/7/21
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备注余小燕(1986-),女,江西人,硕士研究生,研究方向为手性化合物的分离研究。
引用该论文: YU Xiao-yan,GU Xiao-juan,XIE Yu,QU Shang-lan,SHEN Bao-chun. Separation of Enantiomers of 6 Alkamine Drugs on 1-Napthalene Vancomycin Chiral Stationary Phase[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2012, 48(8): 884～886
余小燕,谷晓娟,谢宇,屈尚蓝,沈报春. 六种氨基醇药物在1-萘万古霉素手性固定相上的对映体分离[J]. 理化检验－化学分册, 2012, 48(8): 884～886
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参考文献
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【4】刘雪英,王庆伟,李晓晔,等.高效液相色谱手性固定相拆分酒石酸美托洛尔对映体条件的优化［J］.解放军药学学报, 2005,21(3):186-188.
【5】蔡梅,李忠红,杨丹.硫酸沙丁胺醇的手性分离［J］.药物分析杂志, 2006,26(2):159-161.
【6】李芳,李佳杨,张华燕,等.直链淀粉手性固定相高效液相色谱法拆分比索洛尔对映体［J］.色谱, 2008,26(6):766-768.
【7】徐贝佳,张大同,沈报春,等.7种氨基醇类药物在替考拉宁柱上的对映体分离［J］.分析化学, 200,35(1):55-60.
【8】SHEN Bao-chun, ZHANG Da-tong, YUAN Jian-yong, et al. Evaluation and comparison of a 3,5-dimethylphenyl isocyanate teicoplanin and phenyl isocyanate teicoplanin chiral stationary phases［J］. Chinese Journal of Chemistry, 2009,27:628-632.
【9】CHANG S C, RELD I C, CHEN S, et al. Chromatographic mobile phase for cyclodextrin-bonded chiral. stationary phases［J］. Trends Anal Chem, 1993,12(4):144-153.
【10】ARMSTRONG D W, CHEN S, CHANG C, et al. A new approach for the direct resolution of racemic beta adrenergic blocking agents by HPLC［J］. J Liq Chrom, 1992,15(3):545-556.
【11】KARLSSON C, WIKSTROM H, ARMSTONG D W, et al. Enantioselective reversed-phase and non-aqueous capillary electrochromatography using a teicoplanin chiral stationary phase［J］. J Chromatogr A, 2000,897:349-363.
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