Capillary Electrophoretic Chiral Separation of Amino Acids Based on the Combined Use of Vancomycin as Chiral Selector and Segmented Filling Technique
摘 要
结合区段灌注技术,以万古霉素为手性选择剂构建毛细管电泳手性分离体系,研究了氨基酸对映异构体的分离。选择的最佳分离条件为:① 含3 mmol·L-1万古霉素和2 mmol·L-1 HDB的pH 7为200 mmol·L-1硼酸缓冲溶液的分离区段;② 灌注长度为压力3.45 kPa,时间2.5 min。以含2 mmol·L-1 HDB的pH 7为200 mmol·L-1硼酸缓冲溶液为运行缓冲溶液对19种氨基酸衍生物进行手性分离。试验结果显示其中15对氨基酸对映异构体得到了良好分离。
Abstract
Separation of enantiomers of amino acids was studied by the capillary electrophoretic chiral separation system established on the base of combined use of segmented filling technique and vancomycin as chiral selector. The optimum separation conditions were selected as follows: ① a solution of 200 mmol·L-1 H3BO3 buffer of pH 7 containing 3 mmol·L-1 vancomycin and 2 mmol·L-1 HDB was placed in the separation segmented section with the H3BO3 solution of HDB as conveying solution; ② the length of segmented filling was kept under a pressure of 3.45 kPa through a time of 2.5 min. As shown by the experimental results, 15 pairs of the enantiometric amino acids among the 19 derivatives of amino acids tested were well separated.
中图分类号 O657.8
所属栏目 试验与研究
基金项目 国家自然科学基金(81001595);教育部“长江学者和创新团队发展计划”(IRT0871);中央高校基本科研业务费专项资金中央民族大学自主科研项目(0910KYQN67)
收稿日期 2011/7/19
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联系人作者申刚义(sgy@iccas.ac.cn)
备注申刚义(1976-),男,山西长治人,博士,副教授,从事色谱分析及生物传感方法研究。
引用该论文: SHEN Gang-yi,SHE Dong-lai,ZHANG Ai-qin. Capillary Electrophoretic Chiral Separation of Amino Acids Based on the Combined Use of Vancomycin as Chiral Selector and Segmented Filling Technique[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2012, 48(9): 1008~1011
申刚义,佘东来,张爱芹. 万古霉素手性选择剂结合区段灌注技术的氨基酸毛细管电泳手性分离[J]. 理化检验-化学分册, 2012, 48(9): 1008~1011
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参考文献
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【9】MAIER V, PETR J, SEVCK J, et al. Electrokinetic partial filling technique as a powerful tool for enantiomeric separation of DL-lactic acid by CE with contactless conductivity detection[J]. Electrophoresis, 2007,28:1815-1822.
【10】KANG Jing-wu, WISTUBA D, SCHURIG V. Fast enantiomeric separation with vancomycin as chiral additive by co-electroosmotic flow capillary electrophoresis: Increase of the detection sensitivity by the partial filling technique[J]. Electrophoresis, 2003,24:2674-2679.
【11】康经武,梁冰,陆豪杰,等.以万古霉素为手性选择剂的氨基酸对映体的毛细管电泳快速分离[J].分析化学, 1999,27(4):448-452.
【2】POINSOT V, BAYLE C, COUDERC F. Recent advances in amino acid analysis by capillary electrophoresis[J]. Electrophoresis, 2003,24:4047-4062.
【3】QI Li, QIAO Juan, YANG Geng-liang, et al. Enantiomeric separation of alanyl and leucyl dipeptides by capillary electrophoresis with cyclodextrins as chiral selectors[J]. Electrophoresis, 2009,30:2266-2272.
【4】陈义.毛细管电泳技术及应用[M].2版.北京:化学工业出版社, 2006.
【5】LU Xiao-ning, CHEN Yi. Chiral separation of amino acids derivatized with fluoresceine-5-isothiocyanate by capillary electrophoresis and laser-induced fluorescence detection using mixed selectors of b-cyclodextrin and sodium taurocholate[J]. J Chromatogr A, 2002,955:133-140.
【6】ARMSTRONG D W, RUNDLETT K, REID G L. Use of a macrocyclic antibiotic, rifamycin B, and indirect detection for the resolution of racemic amino alcohols by CE[J]. Anal Chem, 1994,66:1690-1695.
【7】RUNDLETT K L, ARMSTRONG D W. Effect of micelles and mixed micelles on efficiency and selectivity of antibiotic-based capillary electrophoretic enantioseparations[J]. Anal Chem, 1995,67(13):2088-2095.
【8】WANG Zhao-yan, WANG Jun-feng, HU Zhi-de, et al. Enantioseparation by CE with vancomycin as chiral selector: Improving the separation performance by dynamic coating of the capillary with poly(dimethylacrylamide)[J]. Electrophoresis, 2007,28:938-943.
【9】MAIER V, PETR J, SEVCK J, et al. Electrokinetic partial filling technique as a powerful tool for enantiomeric separation of DL-lactic acid by CE with contactless conductivity detection[J]. Electrophoresis, 2007,28:1815-1822.
【10】KANG Jing-wu, WISTUBA D, SCHURIG V. Fast enantiomeric separation with vancomycin as chiral additive by co-electroosmotic flow capillary electrophoresis: Increase of the detection sensitivity by the partial filling technique[J]. Electrophoresis, 2003,24:2674-2679.
【11】康经武,梁冰,陆豪杰,等.以万古霉素为手性选择剂的氨基酸对映体的毛细管电泳快速分离[J].分析化学, 1999,27(4):448-452.
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