采用薄层层析法进行定性和用高效液相色谱法测定芦荟食品厂废弃的芦荟皮中的大黄素和芦荟大黄素含量。用Kromasil-C18色谱柱作为分离柱,甲醇-磷酸(85+15)溶液作为测定大黄素的流动相,甲醇-水(80+20)溶液作为测定芦荟大黄素的流动相,在254 nm处进行检测。大黄素和芦荟大黄素的质量分别在3.75~300 mg和2.5~200 mg范围内与其峰面积呈线性关系,平均回收率分别为96.3%,98.5%,相对标准偏差(n=5)分别为1.3%和1.9%。高效液相色谱法用于测定芦荟食品厂废弃的芦荟皮中大黄素和芦荟大黄素的平均质量分数分别为71,28 mg·kg-1。

TLC and HPLC were applied to the analysis (both qualitative and quantitative) of husk of aloe (which was disposed of as waste from aloe food factory) for the constituents of emodin and aloe-emodin. Kromasil-C18 column was used as stationary phase, the mixture of methanol and H3PO4 (85+15) was used as mobile phase for emodin and mixture of methanol and water (80+20) for aloe-emodin. UV-detection was made at the wavelength of 254nm. Linear relationships between values of peak area and mass were kept in the ranges of 3.75-300 mg for emodin and 2.5-200 mg for aloe-emodin. Values of recovery and RSD′s (n=5) were found 96.3% and 1.3% for emodin, 98.5% and 1.9% for aloe-emodin respectively. Husk of aloe obtained as waste from aloe food factory, was pre-treated and analyzed by HPLC for contents of emodin and aloe-emodin as described by the proposed method, average contents of emodin and aloe-emodin found were 71 mg·kg-1 and 28 mg·kg-1 respectively.