氢化物发生-原子荧光光谱法测定茶叶总硒时样品前处理方法的比较
On Methods of Sample Pretreatment in HG-AFS Determination of Total Selenium in Tea Leaves
摘 要
在氢化物发生-原子荧光光谱法测定茶叶中总硒量时,比较了常规湿法消解(方法1)和微波消解(方法2)两种样品前处理方法。结果表明:从测定精密度而论,方法1略优于方法2,茶叶标准样品(GBW 10016)中硒的测定值的相对标准偏差(n=8)值,方法1为0.35%,方法2为0.52%;从准确度方面,所测得方法1的回收率为94%,方法2的回收率为92%。总体上看,方法1优于方法2,更适合用于氢化物发生-原子荧光光谱法测定茶叶中总硒量。
湿法消解
氢化物发生-原子荧光光谱法
茶叶
硒
Microwave digestion
Wet-digestion
HG-AFS
Tea
Selenium
Abstract
The sample pre-treatment of tea by the method of conventional wet-digestion (method 1) and the method of microwave assisted digestion (method 2) were compared in the HG-AFS determination of total selenium. Value of RSD (n=8) found by method 1 was 0.35%, while by method 2 was 0.52%. Value of recovery found by method 1 was 94%, while by method 2 was 92%. As revealed by the experimental results, better precision and recovery were shown by method 1 than by method 2. On the whole, pretreatment of sample by the conventional wet-digestion was preferred to be used in the HG-AFS analysis.
中图分类号 O657.31
所属栏目 工作简报
基金项目 恩施地区富硒石煤资源综合利用研究(1212011130324)
收稿日期 2011/10/25
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备注陈小磊(1988-),男,湖北荆州人,硕士研究生,主要从事环境分析化学方向研究。
引用该论文: CHEN Xiao-lei,WEI Chang-hua,BAO Zheng-yu,GUO Yu,MA Zhen-zhen,LI Hui. On Methods of Sample Pretreatment in HG-AFS Determination of Total Selenium in Tea Leaves[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2012, 48(9): 1075～1077
陈小磊,魏昌华,鲍征宇,郭宇,马真真,李慧. 氢化物发生-原子荧光光谱法测定茶叶总硒时样品前处理方法的比较[J]. 理化检验－化学分册, 2012, 48(9): 1075～1077
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参考文献
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【3】陈铭,刘更另.高等植物的硒营养及在食物链中的作用［J］.土壤通报, 1996,27(4):185-188.
【4】周令望,钟学宽,高彦辉,等.低硒致乳鼠心肌细胞凋亡的实验研究［J］.中国地方病学杂志, 1999,18(6):422-424.
【5】武瑞,康世良,徐世文,等.硒对汞致抗氧化系统损伤的拮抗作用［J］.中国兽医杂志, 2001,37(11):6-9.
【6】陈汉杰,欧阳政,蔡端仁,等.硒蛋白及其分离分析［J］.色谱, 1992,10(2):75-78.
【7】CAPELO J L, FERNANDEZ C, PEDRAS B, et al. Trends in selenium determination/speciation by hyphenated techniques based on AAS or AFS［J］. Talanta, 2006,68(5):1442-1447.
【8】SMRKOLJ P, STIBILJ V. Determination of selenium in vegetables by hydride generation atomic fluorescence spectrometry［J］. Anal Chim Acta, 2004,512:11-11.
【9】WIBER C G. Toxieology of selenium: Areview［J］. Clin Toxieol, 1980,17:171-230.
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