压电传感器快速检测牛奶中抗生素残留量
Rapid Determination of Residual Amount of Antibiotic in Milk by Piezoelectric Sensor
摘 要
基于嗜热链球菌繁殖使牛奶黏度、密度发生改变,从而导致压电晶体频率发生改变,据此提出了应用压电传感器,通过测定频率检测时间(FDT)来间接测定牛奶中抗生素残留量的方法。氨苄青霉素、氯霉素、氧氟沙星和硫酸双氢链霉素检出限(3S/N)分别为0.30,0.36,0.29,0.28 μg·L-1。方法用于测定牛奶中抗生素的含量,并加入氯霉素做回收试验,测得回收率在82.0%~90.4%之间。
抗生素
嗜热链球菌
牛奶
Piezoelectric sensor
Antibiotic
S. thermophilus
Milk
Abstract
Based on the changes of dynamic viscosity and density of milk with the breeding of S. thermophilus in milk, which lead to the frequency change of piezoelectric sensor, a new method for the indirect determination of residual amount of antibiotics in milk was proposed using piezoelectric sensor by determination of frequency detection time (FDT). Values of detection limit (3S/N) of ampicillin, chloromycetin, cfloxacin and dihydrostreptomycin sulfate found were 0.30, 0.36, 0.29, 0.28 μg·L-1, respectively. Test for recovery was made on the base of milk samples by addition of chloromycetin standard, giving values of recovery in the range of 82.0%-90.4%.
中图分类号 O652.9
所属栏目 试验与研究
基金项目 2010年江苏省高等学校大学生实践创新训练项目(苏高教(2010)16号)
收稿日期 2012/1/16
修改稿日期
网络出版日期
作者单位点击查看
联系人作者袁逸飞(wxy62@cslg.cn)
备注袁逸飞(1990-),男,江苏扬州人,研究方向为化学与生物传感技术。
引用该论文: YUAN Yi-fei,SHEN Mei-jian,ZHANG Long,LI Kang,JIN Li,WANG Xue-ying. Rapid Determination of Residual Amount of Antibiotic in Milk by Piezoelectric Sensor[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2013, 49(2): 134～137
袁逸飞,沈美健,张龙,李康,靳力,汪学英. 压电传感器快速检测牛奶中抗生素残留量[J]. 理化检验－化学分册, 2013, 49(2): 134～137
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参考文献
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【12】廖玲,何艳玲,刘沛.嗜热脂肪芽孢杆菌检测牛乳中抗生素残留［J］.食品工业科技, 2010,31(9):348-351.
【13】郑维君,常炜,马文宏.牛奶中抗生素残留量两种检测方法的比较［J］.食品研究与开发, 2010,31(6):158-159.
【14】HE Feng-jiao, ZHOU Juan. A new antimicrobial susceptibility testing method of Escherichia coli against ampicillin by MSPQC［J］. Journal of Microbiological Methods, 2007,68(3):563-567.
【15】周娟,何凤姣.压电传感器在抗生素给药方案中的应用研究［J］.湖北大学学报:自然科学版, 2009,31(3):290-293.
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【17】SHEN Da-zhong, ZHU Wen-hong, NIE Li-hua, et al. Behavior of a series piezoelectric sensor in electrolyte solution［J］. Enzy Microb Anal Chim Acta, 1993,276(1):87-97.
【18】张波,府伟灵,蒋天伦,等.压电石英晶体传感器液相检测的影响因素［J］.第三军医大学学报, 2002,24(1):8-11.
【19】王志强,胡国媛,李志勇,等.微生物抑制法快速检测鲜奶中多种抗生素残留［J］.中国食品卫生杂志, 2008,20(2):139-141.
【2】曾伟.奶牛乳房炎综合防治［J］.四川畜牧兽医, 2009,36(2):47-49.
【3】张琰图,章竹君,孙永华.高效液相色谱化学发光法检测牛奶中残留四环素类化合物的研究［J］.化学学报, 2006,64(24):2461-2466.
【4】张辉.高效液相色谱法测定肉类食品中四环素类抗生素残留［J］.理化检验-化学分册, 2004,40(10):609-610.
【5】CHEN Huai-xi, CHEN Hui, YING Jun, et al. Dispersive liquid-liquid microextraction followed by high-performance liquid chromatography as an efficient and sensitive technique for simultaneous determination of chloramphenicol and thiamphenicol in honey［J］. Anal Chim Acta, 2009,632(1):80-85.
【6】钱鸣蓉,章虎,吴俐勤,等.固相萃取样品-高效液相色谱-串联质谱法快速测定蜂蜜中26种抗生素［J］.理化检验-化学分册, 2010,46(9):1093-1095.
【7】胡炎明,程雪梅,何太平.高效液相色谱-质谱法测定虾中四环素类抗生素残留量［J］.理化检验-化学分册, 2010,46(4):445-447.
【8】KIM N, KIM D K, KIM W Y. Sulfamethazine detection with direct-binding optical waveguide lightmode spectroscopy-based immunosensor［J］. Food Chemistry, 2008,108(2):768-773.
【9】KIM Y K, KIM H. Immuno-strip biosensor system to detect enrofloxacin residues［J］. Journal of Industrial and Engineering Chemistry, 2009,15(2):229-232.
【10】ASKAL H F, REFAAT I H, DARWISH I A , et, al. A selective spectrophotometric method for determination of rosoxacin antibiotic using sodium nitroprusside as a chromogenic reagent［J］. Spectrochimica Acta Part A, 2008,69(4):1287-1291.
【11】柳玲宝,屠一锋.荧光光度法测定抗生素氧氟沙星［J］.苏州大学学报:自然科学, 2000,16(2):72-75.
【12】廖玲,何艳玲,刘沛.嗜热脂肪芽孢杆菌检测牛乳中抗生素残留［J］.食品工业科技, 2010,31(9):348-351.
【13】郑维君,常炜,马文宏.牛奶中抗生素残留量两种检测方法的比较［J］.食品研究与开发, 2010,31(6):158-159.
【14】HE Feng-jiao, ZHOU Juan. A new antimicrobial susceptibility testing method of Escherichia coli against ampicillin by MSPQC［J］. Journal of Microbiological Methods, 2007,68(3):563-567.
【15】周娟,何凤姣.压电传感器在抗生素给药方案中的应用研究［J］.湖北大学学报:自然科学版, 2009,31(3):290-293.
【16】姚守拙.非质量效应压电化学传感的应用与发展［J］.化学通报, 2001(9):533-536.
【17】SHEN Da-zhong, ZHU Wen-hong, NIE Li-hua, et al. Behavior of a series piezoelectric sensor in electrolyte solution［J］. Enzy Microb Anal Chim Acta, 1993,276(1):87-97.
【18】张波,府伟灵,蒋天伦,等.压电石英晶体传感器液相检测的影响因素［J］.第三军医大学学报, 2002,24(1):8-11.
【19】王志强,胡国媛,李志勇,等.微生物抑制法快速检测鲜奶中多种抗生素残留［J］.中国食品卫生杂志, 2008,20(2):139-141.
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