提出了高效液相色谱法测定油脂类保健食品中虾青素含量的方法。样品经乙腈提取,提取液浓缩后,用Dikma C18色谱柱(4.6 mm×250 mm,5 μm)分离,用不同配比的乙腈和水的混合溶液为流动相梯度洗脱,用紫外检测器于波长479 nm处检测。虾青素的质量浓度在30.0~300 mg·L-1范围内与其峰面积呈线性关系,检出限(3S/N)为0.07 mg·L-1。在空白样品中进行加标回收试验,方法的回收率在90.0%~96.7%之间;方法的相对标准偏差(n=6)为2.8%。

HPLC was applied to the determination of astaxanthin in fatty health food. The sample was extracted with acetonitrile and after concentration, the extract was separated on Dikma C18 chromatographic column (4.6 mm×250 mm, 5 μm) with a mixture of acetonitrile and water mixed in different ratio as mobile phase in gradient elution. UV-detection at the wavelength of 479 nm was adopted. Linear relationship between values of peak area and mass concentration of astaxanthin was obtained in the range of 30.0-300 mg·L-1, with detection limit (3S/N) of 0.07 mg·L-1. Test for recovery was made by addition of standard solution to blank sample, values of recovery obtained were in the range of 90.0%-96.7%. Values of RSD (n=6) of the method was 2.8%.