建立了高效液相色谱法测定大豆磷脂类保健品中磷脂酰胆碱含量的方法。样品经丙酮溶解去除油脂类杂质干扰后,离心弃去上层液体,残液用氮气吹干,然后用正己烷-异丙醇(1+1)混合液溶解残渣,以正相硅胶柱(250 mm×4.6 mm,5 μm)为固定相,以乙腈-甲醇-磷酸(80+20+0.6)溶液等度洗脱分离,用紫外检测器于波长205 nm处检测。磷脂酰胆碱的质量浓度在2.136 g·L-1以内与其峰面积呈线性关系。应用此法分析大豆磷脂软胶囊,平均回收率在90.2%~96.1%之间;测定值的相对标准偏差(n=6)在1.2%~3.6%之间。

A method for determination of phosphatidylcholine in health-care food of soybean phospholipids by HPLC was proposed. The sample was dissolved with acetone to eliminate the interference of impurities of fat and oil, after centrifugation, lower liquid layer was taken and evaporated to dryness by N2-blowing. The residue obtained was dissolved with a mixture of n-hexane-isopropanol (1+1) and separated on normal phase silica gel column (250 mm×4.6 mm, 5 μm). A mixture of acetonitrile, methanol and phosphoric acid mixed in the ratio of 80 to 20 to 0.6 (by volume) was used as mobile phase. UV-detection at 205 nm was used in the determination. Linear relationship between values of peak area and mass concentration of phosphatidylcholine was kept in the range within 2.136 g·L-1. The proposed method was used in the analysis of soybean phospholipids capsule, values of average recovery and RSD′s (n=6) found were in the ranges of 90.2%-96.1% and 1.2%-3.6% respectively.