基于在pH 10.2的碱性介质中,用氯化血红素作为模拟酶催化剂,过氧化苯甲酰经对羟基苯乙酸衍生化后生成荧光二聚体,该荧光物质经激发光照射产生荧光,据此提出了荧光光谱法测定面粉中过氧化苯甲酰含量的方法。在激发波长310 nm、发射波长400 nm处,过氧化苯甲酰的质量浓度在0.5~50 mg·L-1范围内与反应体系的荧光强度呈线性关系,方法的检出限(3s/k)为1 mg·kg-1。方法用于面粉中过氧化苯甲酰含量的测定,测得加标回收率为98.0%~102%,相对标准偏差(n=6)为1.6%~2.2%。

Based on the derivatization of benzoyl peroxide by p-hydroxyphenylacetic acid to give a fluorescent dimer in a basic medium of pH 10.2 with hemin as simulated enzymic catalytic agent and on the fluorescence property of the derivate as shown by its fluorescence spectrum at the wavelengths of 310 nm (λex) and 400 nm (λem), a fluorophotometric method for determination of benzoyl peroxide in wheat flour was proposed. Linear relationship was found between values of fluorescence intensity and mass concentration of benzoyl peroxide in the range of 0.05-50 mg·L-1, with detection limit (3s/k) of 1 mg·kg-1. The proposed method was used in the determination of benzoyl peroxide in wheat flour, giving values of recovery ranged from 98.0% to 102%, and RSD′s (n=6) ranged from 1.6% to 2.2%.