1-丁基-3-甲基咪唑盐酸盐-正丙醇与磷酸氢二钾双水相萃取-分光光度法测定阿奇霉素
Spectrophotometric Determination of Azithromycin by Aqueous Two-phase System Extraction from Mixture Containing ［Bmim］Cl, Propanol and K2HPO4
摘 要
以正丙醇(1.0 mL)和1-丁基-3-甲基咪唑盐酸盐(0.5 mL)为萃取溶剂,以磷酸氢二钾(6.375 g)作为分相盐,调节上相溶液的pH为9.0,在303.15 K的温度条件下对抗生素阿奇霉素作双水相萃取。取上、下相溶液各1.00 mL,分别置于5 mL容量瓶中,各加入0.1 mol·L-1盐酸溶液1.5 mL和硫酸(3+1)溶液2.5 mL,以下按盐酸-硫酸分光光度法于波长481.5 nm处,以试剂空白作参比,测量其吸光度,从工作曲线上查得阿奇霉素的含量。方法的萃取率达89.71%。
双水相体系
阿奇霉素
分光光度法
Propanol
Aqueous two-phase system
Azithromycin
Spectrophotometry
Abstract
Azithromycin (AZMC) was separated from its solution by aqueous two-phase system extraction, using 1.0 mL of n-propanol and 0.5 mL of 1-butyl-3-methylimidazol hydrochloride as extraction solvent, and K2HPO4 (6.375 g) as phase-separating salt. The acidity of the upper phase was adjusted to pH 9.0, and the extraction was carried out at a temperature of 303.15 K. An aliquot of 1.0 mL each was taken from the upper and lower phase and phaced into 5 mL volumetric flasks separately, and to each of the flasks, 1.5 mL of 0.1 mol·L-1 HCl and 2.5 mL of H2SO4 (3+1) were added. Amounts of AZMC in both the solutions were determined spectrophotometrically at the wavelength of 481.5 nm (vs. reagent blank), by the HCl-H2SO4 method. Content of AZMC was found from the working curve. Rate of extraction was found attained to 89.71%.
中图分类号 O657.3
所属栏目 试验与研究
基金项目 中央高校基本科研业务费专项资金基金资助项目(CHD2011TD021;CHD2011JC094;CHD2012JC063)
收稿日期 2012/6/27
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备注关卫省(1960-),男,陕西西安人,教授,博士生导师,主要研究方向为水污染控制。
引用该论文: GUAN Wei-sheng,LI Wen-Juan,HAN Juan. Spectrophotometric Determination of Azithromycin by Aqueous Two-phase System Extraction from Mixture Containing ［Bmim］Cl, Propanol and K2HPO4[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2013, 49(8): 906～909
关卫省,黎文娟,韩娟. 1-丁基-3-甲基咪唑盐酸盐-正丙醇与磷酸氢二钾双水相萃取-分光光度法测定阿奇霉素[J]. 理化检验－化学分册, 2013, 49(8): 906～909
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