高效液相色谱法测定蜈蚣草中二苯砷酸
Determination of Diphenylarsinic Acid in Pteris Vittata L. by HPLC
摘 要
建立了高效液相色谱法测定蜈蚣草中二苯砷酸的分析方法.样品采用0.1 mol·L-1磷酸氢二钠溶液振荡提取4 h,高速离心,上清液以Shimadzu VP-ODS反相C18柱为分离柱,以乙腈-0.02 mol·L-1磷酸二氢钾溶液(16+84)作为流动相进行洗脱,检测波长为220 nm.二苯砷酸的质量浓度在0.20~2.0 mg·L-1范围内与其峰面积呈线性关系,方法的检出限(3S/N)为2.8 mg·kg-1.对空白样品进行加标回收试验,加标回收率在98.5%~98.8%之间,测定值的相对标准偏差(n=5)为0.97%~4.6%.方法可应用于蜈蚣草中二苯砷酸的检测.
二苯砷酸
蜈蚣草
HPLC
Diphenylarsinic acid
Pteris vittata L
Abstract
HPLC was applied for the determination of diphenylarsinic acid (DPAA) in Pteris vittata L.. The sample was extracted by oscillation in Na2HPO4 solution of 0.02 mol·L-1 for 4 h. After centrifugation,the supernatant was separated on a Shimadzu VP-ODS reserved-phase C18 column with acetonitrile-KH2PO4 solution of 0.02 mol·L-1 as the mobile phase for gradient elution. UV-detection was made at 220 nm. The linear relationship was found between the peak area and the mass concentration of DPAA was in the range of 0.20-2.0 mg·L-1,with detection limit of 2.8 mg·kg-1. Recovery rates measured by standard addition methods were in the range of 98.5%-98.8%,with RSD (n=5) in the range of 0.97%-4.6%. The method was used to determine DPAA in Pteris vittata L.
中图分类号 O652.63
所属栏目 工作简报
基金项目 国家自然科学基金重点项目(41230858)
收稿日期 2014/8/19
修改稿日期
网络出版日期
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备注冯仕江(1987-),男,广西合浦人,硕士研究生,研究方向为有机污染土壤生物修复.
引用该论文: FENG Shi-jiang,LUO Yong-ming,REN Wen-jie,LIU Fang,WANG A-nan,MA Wen-ting,LI Zhen-gao,YANG Guang-mei,TENG Ying. Determination of Diphenylarsinic Acid in Pteris Vittata L. by HPLC[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2015, 51(3): 321～324
冯仕江,骆永明,任文杰,刘方,王阿楠,马文亭,李振高,杨光梅,滕应. 高效液相色谱法测定蜈蚣草中二苯砷酸[J]. 理化检验－化学分册, 2015, 51(3): 321～324
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【3】ISHIZAKI M,YANAOKA T,NAKAMURA M,et al. Detection bis(diphenylarsine) oxide,dephenylarsinic acid and phenylarsonic acid,compounds probably derived from chemical warfare agents,in drinking well water[J].Journal of Health Science,2005,51(2):130-137.
【4】ISHII K,TAMAOKA A,OTSUKA F,et al. Diphenylarsinic acid poisoning from chemical weapons in Kamisu,Japan[J].Annals of Neurology,2004,56(5):741-745.
【5】朱宝霞,周廷,王宁,等.东北某地日本遗弃在华化学武器污染土壤调查[C].重庆:中国环境科学学会,2008:2129-2132.
【6】管英强,王学峰,王新明,等.日本遗弃在华化学武器污染土壤处理问题[C].武汉:中国环境科学学会,2009:1173-1174.
【7】张福存,王雨山,石奉华,等.日本在华遗弃化学武器埋藏地调查及其污染处置方法研究[J].地球与环境,2011,39(4):567-570.
【8】李善茂,李伟,岳丽君,等.高效液相色谱法分析环境样品中的苯基胂化合物[J].色谱,2005,23(5):545-547.
【9】BABA K,ARAO T,MAEJIMA Y et al. Arsenic speciation in rice and soil containing related compounds of chemical warfare agents[J].Analytical Chemistry,2008,80(15):5768-5775.
【10】ARAO T,MAEJIMA Y,BABA K. Uptake of aromatic arsenicals from soil contaminated with diphenylarsinic acid by rice[J].Environmental Science and Technology,2009,43(4):1097-1101.
【11】鲁胜利,周黎明,刘波,等.日本遗弃化武砷污染土壤的生物修复技术展望[C].重庆:中国环境科学学会,2008:1068-1070.
【12】周廷,朱宝霞,王新明,等.蜈蚣草修复东北某地日本遗弃化学武器砷污染土壤的可行性分析[C].乌鲁木齐:中国环境科学学会,2011:1708-1713.
【13】TU C,MA L Q. Arsenic accumulation in the hyperaccumulator Chinese brake and its utilization potential for phytoremediation[J].Journal of Environmental Quality,2002,31(5):1671-1675.
【14】李士杏.无机砷和二苯砷酸在土壤中吸附机制及蜈蚣草修复效果研究[D].北京:中国科学院,2011:73-78.
【15】李士杏,刘五星,黄玉娟,等.高效液相色谱法测定土壤中化学武器降解产物二苯砷酸[J].土壤学报,2012(2):394-397.
【16】杨丽君,宋晓华,陈丽君,等.高效液相色谱-电感耦合等离子体质谱法同时测定果蔬中6种砷形态[J].分析试验室,2011,30(7):62-66.
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【18】贾炎,黄海,张思宇,等.无机砷和甲基砷在水稻体内吸收转移的比较研究[J].环境科学学报,2012,30(10):2483-2489.
【19】王钊.砷污染土壤-水稻中砷形态的变化特征及其影响因素研究[D].保定:河北农业大学,2013:28-28.
【20】马丽.砷胁迫下3种水生植物砷形态的变化及影响因素[D].昆明:昆明理工大学,2013:13-14.
【21】侯艳霞,刘丽萍,潘浩,等.高效液相色谱-电感耦合等离子体质谱分析大米中砷形态化合物[J].分析试验室,2013,32(10):103-107.
【22】SCHMIDT A C,KUTSCHERA K,MATTUSCH J,et al. Analysis of accumulation,extractability,and metabolization of five different phenylarsenic compounds in plants by ion chromatography with mass spectrometric detection and by atomic emission spectroscopy[J].Chemosphere,2008,73(11):1781-1787.
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