高效液相色谱法测定化妆品中5种荧光增白剂
HPLC Determination of 5 Fluorescent Whitening Agents in Cosmetics
摘 要
采用高效液相色谱法测定化妆品中5种荧光增白剂的含量.样品经甲醇超声提取,以C18色谱柱为分离柱,以甲醇和5 mmol·L-1四丁基硫酸氢铵溶液以不同比例混合的溶液为流动相进行梯度洗脱,用荧光检测器测定.5种荧光增白剂在一定的质量浓度范围内与其峰面积呈线性关系,方法的测定下限(10S/N)在0.1~2.0 μg·g-1之间.以空白样品为基体进行加标回收试验,所得回收率在85.1%~97.1%之间,测定值的相对标准偏差(n=6)在1.4%~5.7%之间.
荧光增白剂
化妆品
HPLC
Fluorescent whitening agents
Cosmetics
Abstract
HPLC was applied to the determination of 5 fluorescent whitening agents in cosmetics.The sample was extracted ultrasonically with methanol.C18 chromatographic column was used as stationary phase with mixtures of methanol and 5 mmol·L-1 tetrabutylammonium hydrogensulfate with different mixing ratios as mobile phase in the gradient elution,and determined with fluorescence detector.Linear relationships between values of peak area and mass concentration of the 5 fluorescent whitening agents were kept in definite ranges,with lower limits of determination (10S/N) in the range of 0.1-2.0 μg·g-1.On the base of blank sample,test for recovery was made by standard addition method;values of recovery found were in the range of 85.1%-97.1%,with RSD′s (n=6) in the range of 1.4%-5.7%.
中图分类号 O652.63
所属栏目 工作简报
基金项目 浙江出入境检验检疫局科研项目(ZK201250)
收稿日期 2014/5/25
修改稿日期
网络出版日期
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联系人作者吕春华(lvch@ziq.gov.cn)
备注吕春华(1981-),女,浙江东阳人,博士,主要从事理化检验工作.
引用该论文: LV Chun-hua,JI Xin-he,SHI Ying-zhu,HOU Jian-bo,XIE Wen. HPLC Determination of 5 Fluorescent Whitening Agents in Cosmetics[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2015, 51(5): 609～612
吕春华,季鑫鹤,史颖珠,侯建波,谢文. 高效液相色谱法测定化妆品中5种荧光增白剂[J]. 理化检验－化学分册, 2015, 51(5): 609～612
被引情况:
【1】黄 薇,朱龙龙,孙文芳,刘祥萍, "高效液相色谱法测定食用菌中10种荧光增白剂",理化检验－化学分册 51, 1576-1579(2015)
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参考文献
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【9】冼燕萍,郭新东,罗海英,等.固相萃取-超高效液相色谱分离测定洗涤用品中4种荧光增白剂[J].色谱,2013,31(2):162-169.
【10】朱永红,肖瑞琪,马丽,等.高效液相色谱法测定纸制品中荧光增白剂[J].理化检验-化学分册,2013,49(11):1377-1379.
【11】CHEN H C,WANG Shu-ping,DING W H.Determination of fluorescent whitening agents in environmental waters by solid-phase extraction and ion pair liquid chromatography-tandem mass spectrometry[J].J Chromatogr A,2006,1102:135-142.
【2】GB/T 5009.78-2003食品包装用原纸卫生标准的分析方法[S].
【3】刘峻,秦紫明,左莹,等.荧光分光光度法检测食品包装用纸中荧光物质迁移[J].纸和造纸,2011,30(3):57-60.
【4】罗冠中,刘祥,汪晓冬,等.荧光分光光度法测定生活用纸制品中的荧光增白剂[J].中国测试,2009,35(4):68-71.
【5】SHU Wei-chuan,DING W H.Determination of fluorescent whitening agents in laundry detergents and surface waters by solid-phase extraction and ion-pair[J].J Chromatography A,2005,1088:218-223.
【6】SHU Wei-chuan,DING W H.Determination of fluorescent whitening agents in infant clothes and paper materials by ion-pair chromatography and fluorescence detection[J].Journal of the Chinese Chemical Society,2009,56(4):797-803.
【7】SANTOS M D L,BATLLE R,SALAFRANCA J,et al.Subcritical water and dynamic sonication-assisted solvent extraction of fluorescent whitening agents and azo dyes in paper samples[J].J Chromatography A,2005,1064:135-141.
【8】SATOSHI M,HIDESHIGE T,DONG-MYUNG K,et al.Three-dimensional distributions of sewage markers in Tokyo Bay water-fluorescent whitening agents (FWAs)[J].Marine Pollution Bulletin,2006,52:281-292.
【9】冼燕萍,郭新东,罗海英,等.固相萃取-超高效液相色谱分离测定洗涤用品中4种荧光增白剂[J].色谱,2013,31(2):162-169.
【10】朱永红,肖瑞琪,马丽,等.高效液相色谱法测定纸制品中荧光增白剂[J].理化检验-化学分册,2013,49(11):1377-1379.
【11】CHEN H C,WANG Shu-ping,DING W H.Determination of fluorescent whitening agents in environmental waters by solid-phase extraction and ion pair liquid chromatography-tandem mass spectrometry[J].J Chromatogr A,2006,1102:135-142.
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