氨基甲磺酸席夫碱-邻菲啰啉镍(Ⅱ)配合物与DNA反应的共振散射光谱的研究及应用
Study and Application of Resonance Light Scattering Spectroscopy Produced by the Reaction of Aminomethylsulfonic Acid Schiff Base-Phen-Ni(Ⅱ) Complex with Deoxyribonucleic Acid
摘 要
氨基甲磺酸席夫碱-邻菲啰啉镍(Ⅱ)配合物与脱氧核糖核酸相互作用的共振散射光谱在311 nm处体系有共振光散射,据此提出了一种测定脱氧核糖核酸的新方法.优化的试验条件如下:① 缓冲溶液的pH为7.5;② 反应温度为37.5 ℃;③ 反应时间为25 min;④ 配合物的用量为1.1 mL.脱氧核糖核酸的线性范围为0.53~7.87 mg·L-1,方法的检出限为0.16 mg·L-1.方法用于人工试样的分析,回收率在99.7%~102%之间,测定值的相对标准偏差(n=9)为1.7%.
氨基甲磺酸席夫碱-邻菲啰啉镍(Ⅱ)配合物
脱氧核糖核酸
Resonance light-scattering spectroscopy
Aminomethylsulfonic acid schiff base-phen-Ni(Ⅱ) complex
Deoxyribonucleic acid
Abstract
The resonance light-scattering spectrum of the reaction of aminomethylsulfonic acid schiff base-phen-Ni(Ⅱ) complex and deoxyribonucleic acid has a resonance peak at 311 nm. Based on this fact,a new method for determination of deoxyribonucleic acid was proposed. The optimized conditions were as follows: ① pH of buffer solution: 7.5;② reaction temperature: 37.5 ℃;③ reaction time: 25 min;④ amount of the complex: 1.1 mL. The linear range of deoxyribonucleic acid was 0.53-7.87 mg·L-1 with detection limit of 0.16 mg·L-1. The proposed method was applied to the analysis of synthetic samples,giving values of recovery in the range of 99.7%-102% and RSD (n=9) of 1.7%.
中图分类号 O657.3 DOI 10.11973/lhjy-hx201506028
所属栏目 工作简报
基金项目 国家自然科学基金资助项目(21266006);广西自然科学基金资助项目(2012GXNSFAA053034);广西矿冶与环境科学实验中心资助项目(KH2012YB002)
收稿日期 2014/5/16
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联系人作者赖丽燕(lisa4.6@163.com)
备注赖丽燕(1989-),女,江西赣州人,硕士研究生,研究方向为电分析化学.
引用该论文: LAI Li-yan,LIU Zheng,LI Wei,GAO Jiong-yang,HUANG Hong-xia. Study and Application of Resonance Light Scattering Spectroscopy Produced by the Reaction of Aminomethylsulfonic Acid Schiff Base-Phen-Ni(Ⅱ) Complex with Deoxyribonucleic Acid[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2015, 51(6): 847～850
赖丽燕,刘峥,李巍,高炅杨,黄红霞. 氨基甲磺酸席夫碱-邻菲啰啉镍(Ⅱ)配合物与DNA反应的共振散射光谱的研究及应用[J]. 理化检验－化学分册, 2015, 51(6): 847～850
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