Simultaneous Determination of Dopamine,Ascorbic Acid and Uric Acid by Mesoporous Carbon/Gold Nanoparticle Modified Electrode
摘 要
采用溶胶法制备了介孔碳/纳米金复合材料,并利用透射电子显微镜、扫描电子显微镜和X射线衍射仪进行了表征.将合成的复合材料修饰于玻碳电极表面,用循环伏安法同时测定多巴胺(DA)、抗坏血酸(AA)和尿酸(UA).在pH 7.2磷酸盐缓冲溶液中,DA、AA和UA的氧化峰得到了很好的分离;和裸电极比,介孔碳/纳米金修饰电极对DA、AA和UA具有良好的电催化作用,DA、AA和UA的氧化峰的峰电流强度分别增加1.7,2.0,12倍,3种物质的浓度分别在0.20~45.8,4.0~792.0,0.06~166.0 μmol·L-1范围内与其峰电流强度呈线性关系,检出限(3S/N)分别为0.075,7.5,0.021 μmol·L-1.
Abstract
Au nanoparticles were loaded onto the ordered mesoporous carbon CMK-3 by a sol-gel method. The obtained composites were characterized by TEM,SEM and XRD. The Au/CMK-3 modified glassy carbon electrode (GCE) was used to simultaneously detect dopamine (DA),ascorbic acid (AA) and uric acid (UA) by cyclic voltammetry. In a PBS buffer solution (pH=7.2),the oxidation peaks of AA,DA and UA were well separated from each other. The Au/CMK-3 modified electrode displayed excellent electrochemical catalytic activities for DA,AA and UA. The intensity of the oxidation peak currents of DA、 AA and UA on Au/CMK-3 modified electrode was increased by 1.7,2,12 times,respectively,comparing with bare electrode. Linear relationship was found between the intensity of oxidation peak current of DA,AA and UA with the concentration in the range of 0.20-45.8,4.0-792.0,0.06-166.0 μmol·L-1 and the detection limits were 0.075,7.5,0.021 μmol·L-1,respectively.
中图分类号 O657.14 DOI 10.11973/lhjy-hx201507032
所属栏目 专题报道(药物分析)
基金项目 江苏省大学生科研立项(2012JSSPITP1728);国家自然科学青年基金(20905055)
收稿日期 2014/7/17
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联系人作者张钱丽(zqlmhb@163.com)
备注张钱丽(1974-),女,江苏如皋人,副教授,博士,研究方向纳米材料和电催化.
引用该论文: ZHANG Qian-li,XIAO Xin,ZHANG Rong,DAI Peng,ZHOU Shuai,FENG Gang. Simultaneous Determination of Dopamine,Ascorbic Acid and Uric Acid by Mesoporous Carbon/Gold Nanoparticle Modified Electrode[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2015, 51(7): 1018~1022
张钱丽,肖昕,张蓉,戴鹏,周帅,冯刚. 介孔碳/纳米金修饰电极同时测定多巴胺、抗坏血酸和尿酸[J]. 理化检验-化学分册, 2015, 51(7): 1018~1022
被引情况:
【1】张 进,万瑞英,贾宏新, "漫反射傅里叶变换红外光谱法测定注射液中的维生素C",理化检验-化学分册 52, 432-435(2016)
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【2】ZHAO Jie,ZHANG Wei-min,SHERRELL P,et al. Carbon nanotube nanoweb-bioelectrode for highly selective dopamine sensing[J]. ACS Applied Materials & Interfaces,2012,4(1):44-48.
【3】PING Jiang-feng,WU Jian,YING Yi-bin. Development of an ionic liquid modified screen-printed graphite electrode and its sensing in determination of dopamine[J]. Electrochem Commun,2010,12(12):1738-1741.
【4】MALLESHA M,MANJUNATHA R,NETHRAVATHI C,et al. Functionalized-graphene modified graphite electrode for the selective determination of dopamine in presence of uric acid and ascorbic acid[J]. Bioelectrochem,2011,81(2):104-108.
【5】LAKSHMI D,WHITCOMBE M,DAVIS F,et al. Electrochemical detection of uric acid in mixed and clinical samples: A Review[J]. Electroanal,2011,23(2):305-320.
【6】SUN Chia-liang,LEE Hsin-hsien,YANG Jen-ming,et al. The simultaneous electrochemical detection of ascorbic acid,dopamine,and uric acid using graphene/size-selected Pt nanocomposites[J]. Biosens Bioelectron 2011,26(8):3450-3455.
【7】许宇智,苏星鹏,赵欣,等.离子液体/多壁碳纳米管/壳聚糖修饰电极同时检测多巴胺、抗坏血酸及尿酸的研究[J].分析测试学报,2012,30(11):1235-1240.
【8】BI Hua-qing,LI Yan-hui,LIU Shu-feng,et al. Carbon-nanotube-modified glassy carbon electrode for simultaneous determination of dopamine,ascorbic acid and uric acid: The effect of functional groups[J]. Senso Actuat B: Chemi,2012,171/172:1132-1140.
【9】YAO Hong,SUN Yuan-yuan,LIN Xin-hua,et al. Electrochemical characterization of poly (eriochrome black T) modified glassy carbon electrode and its application to simultaneous determination of dopamine,ascorbic acid and uric acid[J]. Electrochim Acta,2007,52(20):6165-6171.
【10】NIU Xiu-li,YANG Wu,GUO Hao,et al. A novel and simple strategy for simultaneous determination of dopamine,uric acid and ascorbic acid based on the stacked graphene platelet nanofibers/ionic liquids/chitosan modified electrode[J]. Talanta,2012,99:984-988.
【11】邓培红,张军,黎拒难.聚氨基乙酸修饰碳黑微电极同时测定多巴胺和抗坏血酸[J].理化检验-化学分册,2009,45(1):7-10.
【12】邓培红,张军,匡云飞,等.普鲁士兰修饰碳黑微电极同时微分伏安法测定多巴胺和抗坏血酸[J].理化检验-化学分册,2006,42(11):933-935.
【13】马伟,田相星,孙登明.铜掺杂聚L-酪氨酸修饰电极同时测定尿酸、多巴胺和抗坏血酸[J].分析试验室,2011,30(12):45-50.
【14】徐国良,李羚,杨光明.金纳米管阵列修饰玻碳电极用于示差脉冲伏安法测定多巴胺[J].理化检验-化学分册,2012,48(12):1470-1473.
【15】黄文华,王百木,张雷.金纳米颗粒在玻碳电极表面的固载及其对抗坏血酸的电催化氧化[J].理化检验-化学分册,2008,44(4):299-303.
【16】张明明,曹小红,黄平弟.自组装纳米金的多壁碳纳米管微电极的制备及其在测定多巴胺中的应用[J].理化检验-化学分册,2012,48(3):253-256.
【17】GIOVANNI M,POH H,AMBROSI A,et al. Noble metal (Pd,Ru,Rh,Pt,Au,Ag) doped graphene hybrids for electrocatalysis[J]. Nanoscale,2012,4(16):5002-5008.
【18】SALGADO J,ALCAIDE F,ALVAREZ G,et al. Pt-Ru electrocatalysts supported on ordered mesoporous carbon for direct methanol fuel cell[J]. J Power Sources,2010,195(13):4022-4029.
【19】RAMOS-SNCHEZ G,BRUNO M,THOMAS Y,et al. Mesoporous carbon supported nanoparticulated PdNi2: A methanol tolerant oxygen reduction electrocatalyst[J]. Int J Hydrogen Energy,2012,37(1):31-40.
【20】XUE Zhong-hua,ZHANG Fan,QIN Dong-dong,et al. One-pot synthesis of silver nanoparticle catalysts supported on N-doped ordered mesoporous carbon and application in the detection of nitrobenzene[J]. Carbon,2014,69:481-489.
【21】JIA Fa-long,YU Xin-xing,ZHANG Li-zhi. Enhanced selectivity for the electrochemical reduction of CO2 to alcohols in aqueous solution with nanostructured Cu-Au alloy as catalyst[J]. J Power Sources,2014,252:85-89.
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