镧-甲基蓝光度法测定硫酸卡那霉素及硫酸新霉素
Spectrophotometric Determination of Kanamycin and Neomycin by Ion-association Reaction with the Binary Complex of La(Ⅲ) and Methyl Blue
摘 要
镧(Ⅲ)-甲基蓝(MB)在酸性条件下与硫酸卡那霉素(KANA)及硫酸新霉素(NEO)反应生成蓝色离子缔合物,两者的最大正吸收波长均位于682 nm,表观摩尔吸光系数为1.71×104L·mol-1·cm-1(KANA)和1.21×104L·mol-1·cm-1(NEO);最大负吸收波长位于620 nm(KANA)和616 nm(NEO),表观摩尔吸光系数为9.57×104L·mol-1·cm-1(KANA)和8.68×104L·mol-1·cm-1(NEO);用双波长法测定时,摩尔吸光系数可达1.10×105L·mol-1·cm-1(KANA)和1.00×105L·mol-1·cm-1(NEO).方法用于市售药物中硫酸卡那霉素及硫酸新霉素含量的测定,结果与标示值相符，回收率在99.8%~100.5%之间.
镧
甲基蓝
硫酸卡那霉素
硫酸新霉素
Spectrophotometry
Ion association complex
Lanthanum(Ⅲ)
Methyl blue
Kanamycin sulfate
Neomycin sulfate
Abstract
In an acetate buffer solution of pH 4.32,the binary complex formed by lanthanum(Ⅲ) and methyl blue reacted with kanamycin (KANA) or neomycin (NEO) to form blue ion association complexes,having positive absorption maxima at 682 nm for both KANA and NEO,with molar absorptivity of 1.71×104L·mol-1·cm-1 (for KANA) and 1.21×104L·mol-1·cm-1 (for NEO),and negative absorption maxima at 620 nm (for KANA) and 616 nm (for NEO),with molar absorptivity of 9.57×104L·mol-1·cm-1 (for KANA) and 8.68×104L·mol-1·cm-1 (for NEO). When the absorption measurement was made by DW-spectrophotometry,the values of molar absorptivity acheved to 1.10×105L·mol-1·cm-1 (for KANA) and 1.00×105L·mol-1·cm-1. In the analysis of a kanamycin injection sample and a neomycin ear drops sample,the results obtained were in consistency with the labelled values,and results of recovery tests were in the range of 99.8%-100.5%.
中图分类号 O657.3 R978.1
所属栏目 工作简报
基金项目 重庆市教委基金资助项目(No.KJ051304)
收稿日期 2005/4/26
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备注江虹(1956-),女,四川资中人,教授,主要从事分析化学教学及科研工作.
引用该论文: JIANG Hong,LIU Yan. Spectrophotometric Determination of Kanamycin and Neomycin by Ion-association Reaction with the Binary Complex of La(Ⅲ) and Methyl Blue[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2007, 43(1): 54～56
江虹,刘艳. 镧-甲基蓝光度法测定硫酸卡那霉素及硫酸新霉素[J]. 理化检验－化学分册, 2007, 43(1): 54～56
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参考文献
【1】中华人民共和国药典委员会编.中华人民共和国药典(第二部)[M].北京:化学工业出版社,2000:900．
【2】中国药物大全编委会.中国药物大全:西药卷[M].北京:人民卫生出版社,1991:326．
【3】袁成,贾暖,王景祥,等.高效液相色谱-间接光度检测法同时测定血清和尿中5种氨基糖苷类抗生素[J].药物分析杂志,1999,19(2):108-110．
【4】Rizk M,EL-Shabrawy Y,Zakhari N A,et al. Fluorimetric determination of aminoglycoside antibiotics using lanthanide probe ion spectroscopy[J]. Talanta,1995,42(12):1849-1856．
【5】江虹,刘绍璞,胡小莉,等.依文思蓝-氨基糖苷类抗生素的显色反应及其分析应用[J].分析化学,2003,31(9):1053-1057．
【2】中国药物大全编委会.中国药物大全:西药卷[M].北京:人民卫生出版社,1991:326．
【3】袁成,贾暖,王景祥,等.高效液相色谱-间接光度检测法同时测定血清和尿中5种氨基糖苷类抗生素[J].药物分析杂志,1999,19(2):108-110．
【4】Rizk M,EL-Shabrawy Y,Zakhari N A,et al. Fluorimetric determination of aminoglycoside antibiotics using lanthanide probe ion spectroscopy[J]. Talanta,1995,42(12):1849-1856．
【5】江虹,刘绍璞,胡小莉,等.依文思蓝-氨基糖苷类抗生素的显色反应及其分析应用[J].分析化学,2003,31(9):1053-1057．
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