高效液相色谱法测定灭蟑膏中氯蜱硫磷
HPLC Determination of Chlorpyrifos in Cockroach Killing Ointment
摘 要
建立了灭蟑膏中1%氯蜱硫磷的超声萃取前处理方法和高效液相色谱分析方法.考查了萃取温度和萃取时间对萃取量的影响.采用μ Bondapak C18(150 mm×3.9 mm,5 μm)色谱柱;以乙腈、水、冰乙酸的体积比为82∶17.5∶0.5为流动相;流速1.0 mL·min-1;检测波长280 nm.相对标准偏差为1.8%,回收率在97%~105%之间.
灭蟑膏
氯蜱硫磷
超声萃取
HPLC
Ultrasonic extraction
Chlorpyrifos
Cockroach killing ointment
Abstract
HPLC was applied to the determination of chlorpyrifos (~1%) “cockroach killing ointment” with a pretreatment of the sample by ultrasonic extraction.The effect of temperature and time on the rate of extraction were studied.The μ-Bondapak C18 (150 mm×3.9 mm,5 μm) was used as chromatographic column,and a mixed solution containing acetonitrile,acetic acid and water in the ratio of 82 to 0.5 to 17.5 (by volume) was used as the mobile phase,passing through the column with a flow-rate of 1.0 mL·min-1,UV-detection at the wavelength of 280 nm was used in the measurements.Tests for precision and recovery were made,and the values of RSD (n=6) obtained was 1.8% and of recovery in the range of 97%-105%,which were comparable with the gas chromatographic method.
中图分类号 O657.37
所属栏目 试验与研究
基金项目
收稿日期 2005/12/12
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备注刘绍从(1965-),男,河北滦南人,高级工程师,博士研究生,主要从事农药、化学危险品的检测研究.
引用该论文: LIU Shao-Cong,SHANG Wei,Lv Gang,LIU Jun,MA Jun. HPLC Determination of Chlorpyrifos in Cockroach Killing Ointment[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2007, 43(2): 96～97
刘绍从,尚为,吕刚,刘军,马军. 高效液相色谱法测定灭蟑膏中氯蜱硫磷[J]. 理化检验－化学分册, 2007, 43(2): 96～97
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参考文献
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