离子色谱法测定菊芋和菊苣中菊糖
Ion Chromatographic Separation and Determination of Inulenin in Tuber of Jerusalem Artichoke and Chicory Root with Pulsed Amperometric Detection
摘 要
研究了采用离子色谱分离各种不同来源菊糖的方法,该方法能够检出1 mg·L-1含量的目标糖,能够区分组成只有一个单糖单位差异的果糖同系物.
菊糖
菊寡糖
菊芋
菊苣
Ion chromatography
Inulenin
Inulenin oligosaccharide
Jerusalem artichoke
Chicory
Abstract
A laboratory method of separation and determination of inulenin in root tuber and stem tuber of Jerusalem artichoke or chicory by ion chromatography with pulsed amperometric detection was established. Detection limit of 1 mg·L-1 was found for the target sugar. Differentiation of fructose homologues having structural differences of one monosaccharide unit was achieved by the proposed method.
中图分类号 O657.37
所属栏目 工作简报
基金项目 “863”计划(2001AA246041和2004AA246040);国家自然科学基金(39970549);“948”计划(2001-244);农业部农业科技成果转化示范项目(农业部农计函(2003)165号).
收稿日期 2006/1/12
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备注王建华(1961-),男,湖北人,研究员,博导,研究方向为糖生物学与生物技术.
引用该论文: WANG Jian-hua,ZHANG Fan,TENG Da,YANG Ya-lin. Ion Chromatographic Separation and Determination of Inulenin in Tuber of Jerusalem Artichoke and Chicory Root with Pulsed Amperometric Detection[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2007, 43(7): 558～560
王建华,张帆,滕达,杨雅麟. 离子色谱法测定菊芋和菊苣中菊糖[J]. 理化检验－化学分册, 2007, 43(7): 558～560
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参考文献
【1】Yokota A,Enomoto K,Tomita F. Purification and properties of an inulin fructotransferase (depolymerizing) by Arthrobacter sp. H65-7[J]. J Ferment Bioeng,1991,72(4):262-265．
【2】Yokota A,Hirayama S,Enomoto K,et al. Production of inulin fructotransferase (depolymerizing) by Arthrobacter sp. H65-7 and preparation of DFA Ⅲ from inulin by the enzyme[J]. J Ferment Bioeng. 1991,72(4):258-261．
【3】Rassi E El. Carbohydrate analysis: high performance liquid chromatography and capillary electrophoresis[M]. Amsterdam: Elsevier Science Press,1994:391-429．
【4】Chatterton N J,Harrison P A,Thornley W R,et al. Purification and quantification of kestoses (fruct-osylsucroses) by gel permeation and anion exchange chromatography[J]. Plant Physiol Biochem,1989,27:289-295．
【5】Chatterton N J,Harrison P A,Thornley W R,et al. Separation and quantification of fructan (inulin) oligomers by anion exchange chromatography[M]. In A. Fuchs (ed) :Inulin and Inulin-containing Crops. Elsevier Scientific Publishers B V,1993:93-99．
【6】Hotchkiss Jr A T,Hicks K B. Analysis of oligogalacturonic acids with 50 or fewer residues by high performance anion-exchange chromatography and pulsed amperometric detection[J]. Anal Biochem,1990,178:200-206．
【7】Rocklin R D,Pohl C A. Determination of carbohydrates by anion exchange chromatography with pulsed amperometric detection[J]. Liq Chromatogar,1983,6:1577-1590．新书介绍
【2】Yokota A,Hirayama S,Enomoto K,et al. Production of inulin fructotransferase (depolymerizing) by Arthrobacter sp. H65-7 and preparation of DFA Ⅲ from inulin by the enzyme[J]. J Ferment Bioeng. 1991,72(4):258-261．
【3】Rassi E El. Carbohydrate analysis: high performance liquid chromatography and capillary electrophoresis[M]. Amsterdam: Elsevier Science Press,1994:391-429．
【4】Chatterton N J,Harrison P A,Thornley W R,et al. Purification and quantification of kestoses (fruct-osylsucroses) by gel permeation and anion exchange chromatography[J]. Plant Physiol Biochem,1989,27:289-295．
【5】Chatterton N J,Harrison P A,Thornley W R,et al. Separation and quantification of fructan (inulin) oligomers by anion exchange chromatography[M]. In A. Fuchs (ed) :Inulin and Inulin-containing Crops. Elsevier Scientific Publishers B V,1993:93-99．
【6】Hotchkiss Jr A T,Hicks K B. Analysis of oligogalacturonic acids with 50 or fewer residues by high performance anion-exchange chromatography and pulsed amperometric detection[J]. Anal Biochem,1990,178:200-206．
【7】Rocklin R D,Pohl C A. Determination of carbohydrates by anion exchange chromatography with pulsed amperometric detection[J]. Liq Chromatogar,1983,6:1577-1590．新书介绍
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