建立了一种测定鸡肉中氧氟沙星、诺氟沙星、环丙沙星、恩诺沙星兽药残留的高效液相色谱法.样品经乙腈提取,正己烷液-液分配去除蛋白和脂类物质,旋转蒸发除去乙腈,乙腈与水溶解残渣,过微孔滤膜,采用高效液相色谱荧光检测,外标法定量.氧氟沙星、诺氟沙星、环丙沙星、恩诺沙星的质量浓度在0.05~1.2 mg·L-1范围内呈线性关系,相关系数为0.999 7~0.999 9.在0.05~1.00 mg·kg-1质量浓度范围内,平均加标回收率在78.3%~101.8%之间,相对标准偏差为3.89%~12.43%.方法的检出限氧氟沙星为0.003 7 mg·kg-1、诺氟沙星为0.003 7 mg·kg-1、环丙沙星为0.003 5 mg·kg-1、恩诺沙星为0.002 2 mg·kg-1.

A HPLC method for the determination of 4 residual quinolone antimicrobials (ofloxacin,norfloxacin,ciprofloxacin and enrofloxacin) in chicken was proposed.The residual medicines in chicken sample were extracted with acetonitrile,protein and fatty substances in the acetonitrile extract were removed by liquid-liquid partition with n-hexane.The acetonitrile layer was collected and evaporated with a rotary evaporator.Finally the residue was taken up with acetonitrile-water (6+4) mixture and used for HPLC determination with fluorescence detector and external standard calibration curve method.Linear relationship between chromatographic peaks and mass concentrations of all the 4 antimicrobials were kept in the range of 0.05-1.2 mg·L-1,with correlation coefficients from 0.999 7 to 0.999 9.Average values of recovery tested in the concentration range of 0.05-1.00 mg·kg-1 were in range from 78.3% to 101.8%,with RSD′s (n=7) from 3.89% to 12.43%.Detection limits of this method were found to be 0.003 7 mg·kg-1 (for ofloxacin,norfloxacin),0.003 5 mg·kg-1 (for ciprofloxacin) and 0.002 2 mg·kg-1 (for enrofloxacin).