应用日立L-8800型全自动氨基酸分析仪测定了药酒中牛磺酸的含量及其稳定性.对仪器的分析条件作了两项调整,其一,梯度淋洗中淋洗剂B2的淋洗时间改为6.0 min;其二,自动进样器的进样模式改为“cut”模式.制备了3种浓度水平牛磺酸含量的药酒样品进行试验.每一试样又分为两份,一份在室温保存,另一份置于冰箱中在4 ℃保存.每份试样在制备的当时即按规定方法测定其牛磺酸含量,之后每隔1个月分析一次,连续测定3个月.测定结果证明,在药酒基体中,牛磺酸稳定性相当好.对同一试样分别进行5次测定,考核了方法的精密度,测得其RSD值为0.74%.测得此方法的回收率在97.3%至105.5%之间.

An aminoacid auto-analyzer (Model Hitachi L8800) was used in the determination of stability and content of taurine in medicinal liquor with 2 modifications in the analytical conditions: ① the time of gradient elution with eluant B2 was set for 6.0 min instead of 7.0 min;and ② the mode of sample introduction of the automatic sampler was changed to the mode of “cut”.In the testing,samples with taurine at 3 different concentration levels were prepared,and each sample was separated into 2 portions,one of which was kept at room temperature while the other portion kept at 4 ℃ in a refrigerator.Content of taurine in each portion of the samples were determined,firstly at the time right after preparation,and then determined successively after one month,two month and three month.As shown by the analytical results,taurine was kept quite stable in the liquor matrix.Precision of the method was tested on a sample for 5 determinations,the content of taurine found were 12.31 mg per 100 mL,and the value of RSD obtained were 0.74%.Values of recovery found were in the range from 97.3% to 105.5%.