制药废液中氨基酸的分离与测定
Separation and Determination of Amino Acids in Pharmaceutical Waste Liquor
摘 要
为测定维生素B12制药厂排出的废液中氨基酸量,先在所取的废液样品中加入乙酸铅使其中蛋白质及其他大分子有机物产生沉淀并用离心法分离.上层澄清液中的氨基酸通过装有强酸性阳离子交换树脂的交换柱(25 cm×2.5 cm)予以分离,用1 mol·L-1氨水将氨基酸从柱上洗脱.在所得洗脱液中用气相色谱-质谱及傅立叶变换红外光谱法对氨基酸进行定性及定量测定.
气相色谱-质谱联用
傅立叶变换红外光谱
氨基酸
Pharmaceutical waste liquor
Hyphenation of GC and MS
FT-infrared spectrometry
Amino acids
Abstract
Waste liquor from a vitamin B12 pharmaceutical factory was treated with lead acetate to precipitate protein and other macro-molecular substances and separated by centrifuging. Amino acids in the supernatant liquid were separated by passing through a column packed with strong-acidic cation exchange resin (26 cm×2.5 cm). Amino acids on the resin were eluted with 1 mol·L-1 ammonia water. Amino acids in the eluate were then analyzed both qualitatively and quantitatively by GC/MS and FT-infrared spectrometry.
中图分类号 O657.37
所属栏目 工作简报
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收稿日期 2006/9/15
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备注刘敬兰(1955-),女,河北辛集人,副教授,研究方向为有机分析.
引用该论文: LIU Jing-lan,HE Shu-mei,ZHANG Hong,WANG Su-hong. Separation and Determination of Amino Acids in Pharmaceutical Waste Liquor[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2008, 44(1): 76～78
刘敬兰,何书美,张宏,王素红. 制药废液中氨基酸的分离与测定[J]. 理化检验－化学分册, 2008, 44(1): 76～78
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参考文献
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