采用毛细管电泳分离莽草酸及其对映体.试验了缓冲溶液的种类、浓度、pH值、分离电压、进样时间、添加剂对分离的影响.结果表明:以30 mmol·L-1三(羟甲基)氨基甲烷-30 mmol·L-1 H3BO3(pH 8.6)为缓冲体系,在波长214 nm,分离电压为15 kV,进样时间为10 s的条件下,实现了对莽草酸及其对映体的分离.

High performance capillary electrophoresis was applied to the separation of enantiomorphs of shikimic acid. Several important factors influential to the separation i.e. pH and concentration of the running buffer solution,working voltage of separation,time of sample introduction and the use of additives,were studied and optimized. Enantiomorphs of shikimic acid were separated satisfactorily under the follwing optimized conditions: ① running buffer solution: 30 mmol·L-1 Tris·HCl+30 mmol·L-1 H3BO3 (pH 8.6);② working voltage of separation: 15 kV;③ wavelength of UV-detection: 214 nm;④ time of sample introduction: 10 s.