高效液相色谱法测定茶叶中茶多酚
HPLC Determination of Polyphenols in Tea
摘 要
应用高效液相色谱法测定了茶叶,特别是绿茶中的8种多酚,包括儿茶素(C)、表没食子儿茶素(EGC)、没食子儿茶素(GC)、表儿茶素(EC)、表没食子儿茶素没食子酸酯(EGCG)、没食子儿茶素没食子酸酯(GCG)、表儿茶素没食子酸酯(ECG)及儿茶素没食子酸酯(CG).在C18反相柱(250 mm×4.6 mm,5 μm)上进行分离,用不同体积比混合的流动相A及流动相B的混合液作梯度淋洗,流速为1.0 mL·min-1,柱温为40 ℃,在280 nm波长处进行紫外检测.上述8组分在0.004~4.0 g·L-1范围内呈线性关系,方法的检出限在0.6~2.6 mg·L-1范围内,平均回收率为83%.
茶多酚
茶叶
HPLC
Tea polyphenols
Tea
Abstract
High performance liquid chromatography (HPLC) was applied to the rapid determination of polyphenols,including catechin (C),epigallocatechin (EGC),gallocatechin (GC),epicatechin (EC),epigallocatechin gallate (EGCG),gallocatechin gallate (GCG),epicatechin gallate (ECG) and catechin gallate (CG),in tea,especially in green tea.The polyphenols were separated on the C18 RP-column (250 mm×4.6 mm,5 μm) by gradient elution using mixed solutions of mobile phase A and mobile phase B in different proportions.Mobile phase A was a solution of methanol and formic acid mixed in the ratio of 99.7 to 0.3 by volume,and mobile phase B was a dil.solution of formic acid in water,containg 3 vol.of formic acid in 1 liter of solution.Flow-rate of mobile phase was set as 1.0 mL·min-1,and the column temperature of 40 ℃ wa taken.UV-detection at 280 nm was adopted in the determination.Linearity was kept in the range from 0.004-4.0 g·L-1,with detection limits (S/N=3) in the range from 0.6-2.6 mg·L-1,Value of average recovery found was 83%.
中图分类号 O657.37
所属栏目 试验与研究
基金项目
收稿日期 2007/7/7
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备注莫燕霞(1981-),女,杭州人,硕士研究生,研究方向为分析化学.
引用该论文: MO Yan-Xia,HU Bao-xiang,HU Wei,MO Wei-Min. HPLC Determination of Polyphenols in Tea[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2008, 44(7): 593～596
莫燕霞,胡宝祥,胡伟,莫卫民. 高效液相色谱法测定茶叶中茶多酚[J]. 理化检验－化学分册, 2008, 44(7): 593～596
被引情况:
【1】罗建民,李君,王成虎, "福林法测定骆驼蓬不同部位中多酚含量",理化检验－化学分册 49, 232-233(2013)
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参考文献
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【3】Yang G Y,Liao J,Kim K,et al.Inhibition of growth and induction of apoptosis in human cancer cell lines by tea polyphenols[J].Carcinogenesis,1998,19(4):611-616．
【4】Benelli R,Ven R,Bisacchi D,et al.Anti-invasive effects of green tea polyphenol epigallocatech in-3-gallate (EGCG),a natural inhibitor of metallo and serine proteases[J].Biological Chemistry,2002,383(1):101-105．
【5】宋冠群,林金明.茶多酚分析方法研究与最新进展[J].世界科技研究与发展,2004,26(1):41-51．
【6】Soriani M,Evans C R,Tyrrell R M.Modulation of the UVA activation of haem oxygenase,collagenase and cyclooxygenase gene expression by epigallocatechin in human skin cells[J].Febs Letters,1998,439(3):253-257．
【7】Pierce A R,Graham H N,Glassner S,et al.Analysis of tea flavanols by gas chromatography of theirtrrimethylsilyl derivatives[J].Anal Chem,1969,41(2):298-302．
【8】Collier P D,Mallows R.The estimation of flavanols in tea by gas chromatography of their trimethylsilyl derivatives[J].J Chromatogr,1971,57:29-45．
【9】Luthria D L,Jones A D,Donovan J L,et al.GC-MS determination of catechin and epicatechin levels in humanplasma[J].J High Resolut Chromatogr,1997,20(11):621-623．
【10】Ding Mingyu,Yang Haijun,Xiao Shanqing.Rapid,direct determination of polyphenols in tea by reversed-phase column liquid chromatography[J].Journal of Chromatography A,1999(849):637-640．
【11】Joseph J Dalluge,Eryant C Nelson,Jeanice Brown Thomas,et al.Sander,Selection of column and grandient elution system for the separation of catechins in green tea using high-performance liquid chromatography[J].Journal of Chromatography A,1988(793):265-274．
【12】李奕.毛细管区带电泳法测定葡萄籽中儿茶素类化合物[J].色谱,2000,18(6):491-494．
【13】戴军.茶叶及茶多酚中儿茶素的高效液相色谱分析方法研究[J].色谱,2001,19(5):398-402．
【14】王琳,王炯.固相萃取高效液相色谱法测定茶叶中多酚[J].理化检验-化学册,2005,41:738-740．
【15】WANG Junde,SHANG Zhenhua,YU Yunlu.Highperformance liquid chromatography[M].Beijing: China Petro Chem Press,1992:130-132．
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