提出了高效液相色谱法测定食品中纳他霉素的方法,样品经甲醇-水(3+1)溶液提取净化后,用高效液相色谱配紫外检测器在305 nm波长处测定.纳他霉素的检测采用保留时间定性,外标法定量.方法的回收率在95.7%~102.0%之间,相对标准偏差均小于3%.方法的检出限(3S/N)为0.50 μg·g-1(固体样品),0.25 mg·L-1(液体样品).

A HPLC method for the determination of natamycin in food was studied and reported in this paper.Natamycin in sample was extracted with methanol-water (3+1) at pH 4.5,natamycin in the extract was then determined by HPLC detected at 305 nm using an UV detector.Qualitative analysis was based on retention time and quantitative determination based on calibration curve.Recovery was in range of 95.7%-102.0%.RSD′s were less than 3%.Detection limit of the method was 0.50 μg·g-1 for solid sample and 0.25 mg·L-1 for liquid sample.