采用高效液相色谱法测定了松节油深加工产物中松油醇的含量,以Waters porasil硅胶柱为固定相,正己烷-乙酸乙酯(99+1)混合溶液为流动相,检测波长为238 nm,外标法定量,在室温条件下分离α-松油醇的左旋、右旋异构体及其他组分,并且准确测定左旋α-松油醇及右旋α-松油醇含量.线性范围分别为0.61~11.20 g·L-1和0.8~13.0 g·L-1,回归方程分别为A=0.806+15.90 C,相关系数为0.999 8和A=0.108 6+17.63 C,相关系数为0.999 7,相对标准偏差分别为1.54%和1.06%,回收率分别为90%~112%和93%~112%.

HPLC was applied to the analysis of deep-processing products of terpentine oil.Enantiotropic isomers of (-)α-terpineol and (+)α-terpineol and some other component were separated on the chromatographic column of waters porasil (4.6 mm×300 mm) under room temperature.A mixture composed of n-hexane and ethylacetate (99+1 by volume) was used as mobile phase.UV-detector at 238 nm was used in the measurement and external standard method was used in the determination.Linear relationships between the absorbance and concentration of terpineols were obtained in the ranges of 0.61-11.20 g·L-1 for (-)α-terpineol,and 0.8-13.0 g·L-1 for (+)α-terpineol,corresponding to the regression equations of A=0.806+15.90 C (r=0.999 8) and A=0.108 6+17.63 C (r=0.999 7) respectively.Precision and recovery were tested,giving values of RSD′s (n=5) 1.54% and 1.06% respectively.Values of recovery of the isomers were in the ranges of 90%-112% and 93%-112% respectively.