提出了用同步扫描-双波长荧光分光光度法同时测定肾上腺素(EP)、去甲肾上腺素(NEP)和多巴胺(DA)3种儿茶酚胺类神经递质.试验表明:荧光检测宜选定发射波长(λem)与激发波长(λex)的波长差为70 nm(Δλ=λem-λex)的条件下进行同步扫描.在λem为385.0 nm时DA的荧光信号不受EP和NEP的干扰,而EP和NEP相互的干扰,采用双波长荧光检测模式可消除.选择测定NEP的波长对为470.0 nm(λem,1)和531.8 nm(λem,2),测定EP的波长对为500.0 nm(λ′em,1)和445.6 nm (λ′em,2).测得荧光强度与3种儿茶酚胺浓度在320 μg·L-1(EP),640 μg·L-1(NEP)及160 μg·L-1(DA)内呈线性关系,检出限(3σ/k)依次为0.20,0.97,0.73 μg·L-1.

Synchronous scanning DW-fluorospectrophotometry was proposed for the simultaneous determination of 3 catecholamine neurotransmitters,i.e.,epinephrine (EP) norepinephrine (NEP) and dopamine (DA).It was found that satisfactory results were obtained by setting the wavelength difference (Δλ) between emission wavelength (λem) and excitation wavelength (λex) at 70 nm in the synchronous scanning fluorescence measurements.The fluorescence signals of DA measured at the λem of 385.0 nm were not interfered by EP and NEP,and the co-interference between EP and NEP was satisfactorily eliminated by adopting the mode of dual wavelength measurement.Hence,EP,NEP and DA were determined simultaneously in the same sample solution.The wavelength pairs of 470.0 nm (λem,1) and 531.8 nm (λem,2) as well as 500.0 nm (λ′em,1) and 445.6 nm (λ′em,2) were chosen for the determination of NEP and EP respectively.Linear relationships between values of fluorescence intensity and concentration of the catecholamines were found in the ranges within 320 μg·L-1 (for EP),640 μg·L-1 (for NEP) and 160 μg·L-1 (for DA),and the detection limits (3σ/k) found were 0.20,0.97,0.73 μg·L-1 respectively.