采用甲醇超声提取法提取农吉利中农吉利甲素,用高效液相色谱法(HPLC)测定农吉利甲素的含量.用L9(34)正交试验设计优化了提取溶剂、提取温度、提取时间、料液比等试验条件;以甲醇-三乙胺(0.5+99.5)混合溶液(pH 7.0)为流动相,对生物碱提取液进行了色谱分离,农吉利甲素质量浓度在1.0~800 mg·L-1范围内呈线性关系,方法的检出限(3S/N)为0.1 mg·L-1,加标回收率试验的结果在97.8%~102.3%之间.

Monocrotaline was separated from sample of crotalaria sessiliflora by ultrasonic extraction with methanol.Influential factors of extraction,including the extraction solvents,time and temperature of extraction as well as the ratio of mass of sample to volume of extraction solvent,were studied and optimized by the method of orthogonal design with L9(34) orthogonal layout.In the HPLC determination,UltimateTM C18 (4.6 mm×250 mm,5 μm) column was used as stationary phase and a mixture of methanol and triethylamine-water (0.5+99.5,pH 7.0) solution,mixed in the ratio of 30 to 70,was used as mobile phase.Linear relationship between values of peak area and concentration of monocrotaline was obtained in the range of 1.0-800 mg·L-1.Detection limit (3S/N) found for the method was 0.1 mg·L-1.Recovery was tested by standard addition method and values of recovery found were ranged from 97.8% to 102.3%.