食用菌样品经乙酸乙酯提取、氮气吹干,0.05 mol·L-1盐酸溶解后,经固相萃取小柱净化用液相色谱法测定.测定中采用Waters Symmetry C18色谱柱为固定相以及磷酸盐缓冲溶液和乙腈(75+25)混合溶液作为流动相.用二极管阵列紫外检测器于288 nm波长处进行紫外检测,外标法定量.方法的检出限(3S/N)和测定下限(10S/N)依次为0.010 mg·L-1和0.025 mg·L-1.在4个不同的浓度水平对方法的回收率进行了试验,测得结果在72.0%~86.0%之间.测定值的相对标准偏差(n=6)均小于8.5%.

Residual amounts of thiabendazole (TBD) and carbendazim (CBD) were extracted ulltrasonically with ethylacetate from samples of edible fungus.The extract was evaporated to dryness by N2-blowing and the residue was dissolved in 0.05 mol·L-1 HCl.TBD and CBD in dil.HCl solution was then purified by SPE.The Waters Symmetry C18 column was used as stationary phase and a mixture of phosphate buffer solution and acetonitrile (mixed in the ratio of 75+25 by volume) was used as mobile phase.UV-detection was made using diode array detector at the wavelength of 288 nm,and external standard were used for quantification.Limit of detection (3S/N) and lower limit of determination (10S/N) of the methd were found to be 0.010 mg·L-1 and 0.025 mg·L-1 respectively for both bactericides.Tests for recovery and precision were made at 4 different concentration levels,and values of recovery found were in the range of 72.0%-86.0% with values of RSD′s (n=6) less than 8.5%.