萃取方法对气相色谱-质谱法测定海洛因滥用者血液中代谢物的影响
Effect of Extraction Methods in GC-MS Determination of Metabolites in Blood of Herion-Abuser
摘 要
研究了海洛因滥用者血液萃取过程中液相萃取和固相萃取方法对其及其代谢物检测结果的影响.以乙基吗啡为内标,以气相色谱-质谱为检测手段,检测前采用乙酰化处理,比较了血液检材中吗啡、6-单乙酰吗啡(6-MAM)、可待因的两种萃取方法得到的结果;试验了海洛因的中间代谢产物6-单乙酰吗啡在萃取中的水解率.结果表明:固相萃取中6-单乙酰吗啡的水解率高于液相萃取,导致6-单乙酰吗啡的回收率和检测灵敏度降低.鉴于6-MAM为判别滥用海洛因的重要指标和有力证据,在海洛因滥用者血液的分析中宜选择液相萃取方法.
海洛因代谢物
液相萃取
固相萃取
6-单乙酰吗啡
GC-MS
Metabolites of heroin
Solvent extraction
SPE
6-Monoacetylmorphine
Abstract
In the GC-MS determination of metabolites of heroin,especially morphine,6-monoacetyl morphine (6-MAM) and codeine,in blood of heroin-abuser,effect of methods by solvent extraction (SVE) and by solid phase extraction (SPE) on the analytical results was studied.Pre-derivatization by acetylation and using ethyl morphine as internal standard were applied in the analysis.It was shown by the analytical results of the 3 metabolites mentioned above,and by the rate of hydrolysis of 6-MAM during the process of extraction,the rate of hydrolysis of 6-MAM in SPE was found to be higher than those in SVE,thus leading to decrease of recovery and detection limit by using SPE in extraction.As the content of 6-MAM is the important index and powerful evidence for the abuse of heroir,the extraction by SVE was more suitable to be used for the GC-MS determination of metabolites in blood of heroin-abuser.
中图分类号 O657.63
所属栏目 试验与研究
基金项目 北京市教育委员会共建项目建设计划资助(JD100410669);国家“十一五”科技支撑项目(2006BAK09B06)
收稿日期 2009/1/8
修改稿日期
网络出版日期
作者单位点击查看
备注孟品佳(1955-),女,辽宁人,教授,博士,研究方向为毒品分析.
引用该论文: MENG Pin-jia,WANG Yan-yan,WANG Ji-fen. Effect of Extraction Methods in GC-MS Determination of Metabolites in Blood of Herion-Abuser[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2009, 45(11): 1249～1253
孟品佳,王燕燕,王继芬. 萃取方法对气相色谱-质谱法测定海洛因滥用者血液中代谢物的影响[J]. 理化检验－化学分册, 2009, 45(11): 1249～1253
共有人对该论文发表了看法，其中：
人认为该论文很差
人认为该论文较差
人认为该论文一般
人认为该论文较好
人认为该论文很好
参考文献
【1】DRUID H,STRANDBERG J J,ALKASS K,et al.Evaluation of the role of abstinence in heroin overdose deaths using segmental hair analysis[J].Forensic Science International,2007,168:223-226．
【2】SCHEIDWEILER K B,HUESTIS M A.Simultaneous quantification of opiates,cocaine,and metabolites in hair by LC-APCI-MS/MS[J].Anal Chem,2004,76:4358-4363．
【3】KRAEMER T,PAUL L D.Bioanalytical procedures for determination of drugs of abuse in blood[J].Anal Bioanal Chem,2007,388:1415-1435．
【4】GUILLOT E,MAZANCOURT P D,DURIGON M,et al.Morphine and 6-acetylmorphine concentrations in blood,brain,spinal cord,bone marrow and bone after lethal acute or chronic diacetylmorphine administration to mice[J].Forensic Science International,2007,166:139-144．
【5】L PEZ P,BERMEJO A M,TABERNERO M J,et al.Determination of cocaine and heroin with their respective metabolites in meconium by gas chromatography-mass spectrometry[J].J Appl Toxicol,2007,27:464-471．
【6】CORDERO R,PATERSON S.Simultaneous quantification of opiates,amphetamines,cocaine and metabolites and diazepam and metabolite in a single hair sample using GC/MS[J].Journal of Chromatography B,2007,850:423-431．
【2】SCHEIDWEILER K B,HUESTIS M A.Simultaneous quantification of opiates,cocaine,and metabolites in hair by LC-APCI-MS/MS[J].Anal Chem,2004,76:4358-4363．
【3】KRAEMER T,PAUL L D.Bioanalytical procedures for determination of drugs of abuse in blood[J].Anal Bioanal Chem,2007,388:1415-1435．
【4】GUILLOT E,MAZANCOURT P D,DURIGON M,et al.Morphine and 6-acetylmorphine concentrations in blood,brain,spinal cord,bone marrow and bone after lethal acute or chronic diacetylmorphine administration to mice[J].Forensic Science International,2007,166:139-144．
【5】L PEZ P,BERMEJO A M,TABERNERO M J,et al.Determination of cocaine and heroin with their respective metabolites in meconium by gas chromatography-mass spectrometry[J].J Appl Toxicol,2007,27:464-471．
【6】CORDERO R,PATERSON S.Simultaneous quantification of opiates,amphetamines,cocaine and metabolites and diazepam and metabolite in a single hair sample using GC/MS[J].Journal of Chromatography B,2007,850:423-431．
相关信息
