HPLC Determination of Phthalates in Cosmetics
摘 要
采用高效液相色谱法测定化妆品中的邻苯二甲酸酯含量.样品用甲醇超声提取,高速离心,上清液经0.45 μm滤膜过滤后,SB-C18 ZORBAX色谱柱(4.6 mm×150 mm,5 μm)分离,甲醇和水为流动相梯度淋洗,在230 nm波长检测.邻苯二甲酸二甲酯(DMP)、邻苯二甲酸二乙酯(DEP)、邻苯二甲酸二丁酯(DBP)、邻苯二甲酸二己酯(DHP)和邻苯二甲酸二辛酯(DOP)的峰面积与其质量浓度均在1~100 mg·L-1范围内呈线性关系,方法的检出限(3S/N)分别为4.0,7.4,4.3,5.2,7.3 ng.5种邻苯二甲酸酯的加标回收率在94.7%~101.1%之间,相对标准偏差(n=6)在0.023%~0.68%之间.
Abstract
HPLC was applied to the determination of phthalates,including dimethyl phthalate (DMP),diethyl phthalate (DEP),dibutyl phthalate (DBP),dihexyl phthalate (DHP),and dioctyl phthalate (DOP) in cosmetics. The sample was ultrasonically extracted with methanol and centrifuged at high speed. The supernatant liquid obtained was filtrated with 0.45 μm membrane,and was separated by gradient elution with methanol and water on SB-C18 ZORBAX column (4.6 mm×150 mm,5 μm). The contents of phthalates were determined at the wavelength 230 nm by external standard method. Linear relationships between the values of peak area and mass concentration of 5 phthalates were kept in the range of 1-100 mg·L-1,with detection limits (3S/N) of 4.0,7.4,4.3,5.2,and 7.3 ng,respectively. The values of recovery found by standard addition method were in the range of 94.7%-101.1%,with RSD′s (n=6) ranged from 0.023% to 0.68%.
中图分类号 O657.7
所属栏目 工作简报
基金项目 上海市科学技术委员会项目(08142200800)
收稿日期 2009/4/27
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备注陈黎明(1976-),男,山东人,工程师,硕士,主要从事色谱和光谱分析工作.
引用该论文: CHEN Li-ming,YAO Ying,WANG Hu,HU Guo-sheng,ZHU Xi-ju. HPLC Determination of Phthalates in Cosmetics[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2009, 45(12): 1399~1400
陈黎明,姚颖,王虎,胡国胜,朱锡菊. 高效液相色谱法测定化妆品中邻苯二甲酸酯[J]. 理化检验-化学分册, 2009, 45(12): 1399~1400
被引情况:
【1】谢宇奇,凌绍明,胡宝英, "藏红T-Fenton体系光度法测定邻苯二甲酸二辛酯的含量",理化检验-化学分册 52, 1332-1334(2016)
【2】马 军,滕 应,马文亭,陆引罡, "层析净化-气相色谱-质谱法测定烟叶中6种邻苯二甲酸酯",理化检验-化学分册 52, 392-396(2016)
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参考文献
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【2】任晋,蒋可.内分泌干扰剂的研究进展[J].化学进展,2001,14(2):135-143.
【3】丁鹏,赵晓松,刘剑锋.酞酸酯类化合物(PAES)研究新进展[J].吉林农业大学学报,1999,21(3):119-124.
【4】BENJAMIN C,BLOUNT K. ERIC M,et al. Quantitative detection of eight phthalate metabolites in human urine using HPLC-APCI-MS/MS[J]. Analy Chem,2000,72(17):4127-4034.
【5】王超英,李碧芳,李攻科.固相微萃取/高效液相色谱联用分析水样中邻苯二甲酸酯[J].分析测试学报,2005,24(5):35-38.
【6】王连珠,王瑞龙,刘溢娜,等.分散固相萃取-气相色谱-质谱法测定罐头食品中6种邻苯二甲酸酯[J].理化检验-化学分册,2008,44(6):502-506.
【7】SHARMAN M,READ W,CASTLE L,et al. Levels of di-(2-ethylhexyl) phthalate and phthalate esters in milk,cream,butter and cheese[J]. Food Additives and Contaminants,1994,11(3):375-385.
【8】俞雄飞,林振兴,莫卫民.傅里叶变换红外光谱法对聚氯乙烯及邻苯二甲酸酯类增塑剂的快速鉴定[J].理化检验-化学分册,2007,43(11):970-972.
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