Separation of Enantiomers of Atenolol by HPLC Using Mobile Phase with Chiral Additive
摘 要
采用C18固定相,以磺丁基醚-β-环糊精为手性流动相添加剂,提出了阿替洛尔对映体的高效液相色谱拆分方法.选用Kromasil C18色谱柱(250 mm×4.6 mm,5 μm),流动相为含20 mmol·L-1磺丁基醚-β-环糊精的pH 8.2的10 mmol·L-1磷酸盐缓冲溶液-甲醇-乙腈(60+35+5),紫外检测波长275 nm,柱温为25 ℃,进样量20 μL,流量为0.5 mL·min-1时,分离度达到1.63.
Abstract
Separation of enantiomers of atenolol by HPLC was proposed using the Kromasil C18 chromatographic column (250 mm×4.6 mm,5 μm) as stationary phase,and sulfobutylether-β-cyclodextrin (SBE-β-CD in short) as chiral agent added to the mobile phase which is a mixture of 10 mmol·L-1 K2HPO4,methanol and acetonitrile mixed in the ratio of 60+35+5 and containing 20 mmol·L-1 of SBE-β-CD.Acidity of the mobile phase was controlled at pH 8.2.UV detection at the wavelength of 275 nm was adopted in the determination,and column temperature was set at 25 ℃.The flow-rate of mobile phase taken was 0.5 mL·min-1.Volume of sample solution introduced was 20 μL.Under the prescribed conditions,value of separation factor achieved was 1.63.
中图分类号 O652.63
所属栏目 工作简报
基金项目 天津科技大学引进人才科研启动基金(0200064)
收稿日期 2008/12/26
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备注周采菊(1963-),女,辽宁沈阳人,硕士,副教授,主要从事分析化学和药物分析研究.
引用该论文: ZHOU Cai-ju,HE De-long. Separation of Enantiomers of Atenolol by HPLC Using Mobile Phase with Chiral Additive[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2010, 46(2): 184~185
周采菊,贺德龙. 高效液相色谱手性流动相添加法拆分阿替洛尔对映体[J]. 理化检验-化学分册, 2010, 46(2): 184~185
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参考文献
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【3】CLAUDIO G,ANNA T,SERGIO C,et al.Simultaneous determination of atenolol and chlorthalidone in plasma by high performance liquid chromatography application to pharmacokinetic studies in man[J].J Chromatogr B,1997,698(1/2):187-194.
【4】BROWN R A,LLG K J,CHEN A F,et al.Dietary Mg2+ supplementation restores impaired vasoactive responses in isolated rat aorta induced by chronic ethanol consumption[J].Eur J Pharmacol,2002,442(3):241-250.
【5】BISWAS N M,RANOJOY S G,ALOK C,et al.Effect of atenolol on cadmium-induced testicular toxicity in male rats[J].Repro Toxicol,2001,15(6):699-704.
【6】杨非,王嗣岑,贺浪冲.β2受体阻滞剂对映体在ChiralcelOD手性柱上的拆分特性[J].西安交通大学学报:医学版,2004,25(2):124.
【7】左文坚.高效液相色谱法手性色谱柱分离β2阻滞剂对映体[J].天津药学,2001,13(6):17.
【8】陈仲益,夏宗玲,胡纯琦,等.手性毛细管电色谱拆分比索洛尔、阿替洛尔、克仑特罗和特布他林[J].分析化学,2007,35(2):181.
【9】安登魁.现代药物分析选论[M].北京:中国医药科技出版社,2001:91.
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