超高效液相色谱-串联质谱法测定染发剂中7种酚类化合物
UHPLC-MS/MS Determination of Seven Aminophenols in Hair Dyes
摘 要
提出了应用超高效液相色谱-串联质谱法同时测定染发剂中4-氨基-2-硝基苯酚、3-二乙氨基酚、2-氨基-4-氯苯酚、2-氨基-5-硝基苯酚、2-氨基-3-硝基苯酚、1,7-二羟基萘酚和2,3-二羟基萘酚等7种酚类化合物的方法.采用甲醇萃取染发剂中酚类成分,经Waters Acquity UPLCTM BEH C18色谱柱分离,外标法定量,多反应监测模式采集质谱数据.7种酚类化合物的检出限(3S/N)均低于50.0 μg·L-1.在10,20,50 μg·g-1三个添加水平下,7种酚类化合物的回收率在68.8%~112.5%之间,相对标准偏差(n=6)在1.58%~12.61%之间.
酚类化合物
萘酚
染发剂
UHPLC-MS/MS
Aminophenols
Naphthol
Hair dyes
Abstract
A method of UHPLC-MS/MS for simultaneous determination of seven aminophenols,including 2-amino-3-nitrophenol,4-amino-2-nitrophenol,2-amino-5-nitrophenol,2-amino-4-chlorophenol,3-diethylaminophenol,1,7-dihydroxynaphthalene and 2,3-dihydroxynaphthalene in hair dyes was proposed.The aminophenols in sample were extracted with methanol,and separated on Waters ACQUITY UPLCTM BEH C18 column and external standard method was used in the determination.The multi-reaction monitoring (MRM) mode was used for MS data collection.The values of detection limits (3S/N) for the 7 phenols found were less than 50.0 μg·L-1.Tests for recovery were carried out at three concentration levels of 10,20,50μg·g-1,giving values of recovery in the range from 68.8% to 112.5% and RSD (n=6) in the range from 1.58% to 12.61%.
中图分类号 O657.63
所属栏目 试验与研究
基金项目 质检总局科研项目(No.2007IK097)
收稿日期 2009/3/5
修改稿日期
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备注于文莲(1964),女,北京人,高级工程师,研究方向为分析化学.
引用该论文: YU Wen-lian,ZHAO Kai-jing,SUN Xin,DU Zhen-xia. UHPLC-MS/MS Determination of Seven Aminophenols in Hair Dyes[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2010, 46(3): 220～223
于文莲,赵开径,孙鑫,杜振霞. 超高效液相色谱-串联质谱法测定染发剂中7种酚类化合物[J]. 理化检验－化学分册, 2010, 46(3): 220～223
被引情况:
【1】吕春华,刘海山,楼成杰,谢文,陈笑梅,何建敏, "高效液相色谱法测定氧化型染发剂中11种染料",理化检验－化学分册 48, 1347-1349(2012)
【2】虞柯洁,周耀明,童红武,刘少民, "高效液相色谱法测定染发剂中α-萘酚",理化检验－化学分册 48, 977-978(2012)
【3】康明芹,李爱军,牟峻,张代辉,胡婷婷,杨帆, "高效液相色谱-串联质谱法同时测定染发剂中的1-萘酚和2-萘酚",理化检验－化学分册 50, 187-190(2014)
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【3】NARITA M,MURAKAMIA K,KAUFFMANN J M.Determination of dye precursors in hair coloring products by liquid chromatography with electrochemical detection[J].Anal Chim Acta,2007(588):316-320．
【4】ZHOU Jian,XU Hong,WAN Guo-hui,et al.Enhancing and inhibiting effects of aromatic compounds on luminal dimethylsulfoxide-OH-chemiluminescence and determination of intermediates in oxidative hair dyes by HPLC with chemiluminescence detection[J].Talanta,2004(64):467-477．
【5】AKYZ M,ATA S.Determination of aromatic amines in hair dye and henna samples by ion-pair extraction and gas chromatography-mass spectrometry[J].Journal of Pharmaceutical and Biomedical Analysis,2008(47):68-80．
【6】于文莲,周新,张青,等.染发剂中2,2-氨基2,4-2硝基苯酚和2,2-氨基2,5-2硝基苯酚的液相色谱-质谱法测定方法研究[J].分析测试学报,2007,26(9):355-359．
【7】RASTOG S C.A method for the analysis of intermediates of oxidative hair dyes in cosmetic products[J].J Sep Sci,2001,24:173-178．
【8】朱会卷,杨艳伟,张卫强,等.高效液相色谱法测定染发剂中的22种染料成分[J].色谱,2008,26(5):554-558．
【9】郑志方.高效液相色谱法测定染发剂中对苯二胺及某些酚类物质的含量[J].香料香精化妆品,2000,7(2):11-14．
【10】李英,刘志红,刘丽.气相色谱-质谱选择离子法测定氧化型染发剂中9种组分[J].香料香精化妆品,2004,6(3):9-12．
【11】VINCENT U,BORDIN G.Validation of an analytical procedure for the determination ofoxidative hairdyes in cosmetic for-mulations[J].J Cosmetic Sci,2002,53:43-58．
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