N-｛3-[3-(二甲基氨基)-1-氧基-2-丙烯基]-苯基｝乙酰胺(NDAOPPAM)样品用流动相甲醇-水(38+62)混合溶液溶解,用Diamonsil C18色谱柱作固定相.在所述条件下,NDAOPPAM与合成反应中的反应物之一,3-乙酰氨基苯乙酮(AAMPAN)可完全分离.定量中用紫外光谱检测,波长为236 nm,测得的峰面积值与NDAOPPAM浓度在40.4~161.6 mg·L-1范围内呈线性关系,检出限(3S/N)为1 ng·L-1.测得方法的日内和日间相对标准偏差(n=6)依次为0.53%和0.89%.在NDAOPPAM产品中残留的未反应完的反应物AAMAPN可用同样方法测定.测得从不同批号产品取得的3个样品中AAMPAN的质量分数分别为0.32%,0.43%,0.49%.

Sample of N-｛3-[3-(dimethylamino)-1-oxo-2-propenyl]-phenyl｝ acetamide (abbreviated as NDAOPPAM) was dissolved and diluted to 100.0 mL with the mobile phase of mixture of methanol and H2O (38+62).Diamonsil C18 chromatographic column was used as stationary phase.Under the conditions described,NDAOPPAM was completed separated from 3-acetamidoacetophenenone (AAMAPN) which is one of the reactants in the synthesis of NDAOPPAM,and determined with ultraviolet detection at wavelength of 236 nm.Linear relationship between values of peak area and concentration of NDAOPPAM was obtained in the range of 40.4-161.6 mg·L-1.Detection limit (3S/N) found was 1 ng·L-1.Values of within-day and inter-day RSD′s (n=6) found for NDAOPPAM were 0.53% and 0.89% respectively.The un-reacted reactant,AAMAPN,remained in the product NDAOPPAM was determined under the same way.Three samples taken from different batches were analyzed and contents of AAMAPN found were 0.32%,0.43% and 0.49% respectively.