高速匀浆提取-高效液相色谱法测定烟草样品中多酚
HPLC Determination of Polyphenols in Tobacco After Extraction by High-Speed Homogenization
摘 要
提出了高效液相色谱法测定烟草样品中多酚含量的方法.烟草样品经甲醇-水(80+20)溶液匀浆提取，提取液用装有MCI-GEL反相树脂的固相萃取小柱脱脂,以Waters XTerra RP-18(3.9 mm×150 mm,5 μm)色谱柱为固定相,乙酸(1+99)和甲醇为流动相梯度洗脱,用二极管阵列检测器于345 nm波长处检测.烟草中主要的植物多酚均达到基线分离,方法加标回收率为95.2%~105.2%,相对标准偏差(n=7)为1.6%~3.0%.
高速匀浆
多酚
烟草
HPLC
High-speed homogenization
Polyphenols
Tobacco
Abstract
A method of HPLC for the determination of polyphenols in tobacco was proposed.The sample was extracted with methanol-H2O(80+20) solution by high-speed homogenization,and the extract obtained was defatted on MCI-GEL reversed phase resin SPE column.The polyphenols were separated by gradient elution with mixed solutions of methanol and acetic acid (1+99) of different ratios on Waters XTerra RP-18 column (3.9 mm×150 mm,5 μm),and determined at the wavelength of 345 nm with diode array detector (DAD).The main plant polyphenols in tobacco were successfully separated,giving values of recovery in the range of 95.2%-105.2% and RSD′s (n=7)in the range of 1.6%-3.0%.
中图分类号 O652.63
所属栏目 试验与研究
基金项目 云南省省院省校科技合作项目(2006YX17);中烟云南工业公司重大专项(2006B309)资助
收稿日期 2009/5/30
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备注徐祎然(1986-),女,云南红河人,硕士生,从事烟草化学研究工作.
引用该论文: XU Yi-ran,XUE Jing-jiao,HE Bin,JIN Yong-can,YANG Guang-yu,HU Qiu-fen. HPLC Determination of Polyphenols in Tobacco After Extraction by High-Speed Homogenization[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2010, 46(4): 351～353
徐祎然,薛景娇,贺宾,金永灿,杨光宇,胡秋芬. 高速匀浆提取-高效液相色谱法测定烟草样品中多酚[J]. 理化检验－化学分册, 2010, 46(4): 351～353
被引情况:
【1】李银科,舒丽丹,杨海英,叶艳青,李明, "高效液相色谱法测定竹叶兰中9种活性成分含量",理化检验－化学分册 50, 312-316(2014)
【2】高燕,杨丽英,马银海, "高效液相色谱法测定玫瑰花中9种黄酮",理化检验－化学分册 50, 353-356(2014)
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【6】李福娟,蔡文生,邵学广.反相高效液相色谱法分离测定烟草中的多酚类化合物[J].色谱,2007,25(4):565-568．
【7】陈子雷,张红,王文博,等.气相色谱法测定高脂类食品中山梨酸、苯甲酸的含量[J].中国卫生检验杂志,2006,16(12):1228-1231．
【8】BIRADAR S V,PATIL A R,SUDARSAN G V,et al.A comparative study of approaches used to improve solubility of roxithromycin[J].Powder Technology,2006,169(1):22-32．
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