HPLC Determination of 10 Fluorescent Whitenings in Mushrooms
摘 要
采用高效液相色谱法同时测定食用菌中10种双三嗪氨基二苯乙烯型荧光增白剂的含量。色谱分离中,以Athena C18-WP色谱柱(4.6 mm×200 mm,5 μm)为固定相,用乙腈和0.02 mol·L-1乙酸铵溶液以不同比例混合的溶液为流动相进行梯度洗脱,用荧光检测器测定,激发波长为350 nm,发射波长为430 nm。10种荧光增白剂的质量浓度均在2.0~100 μg·L-1范围内与其峰面积呈线性关系,方法的检出限(3S/N)在0.002~0.005 mg·kg-1之间。以空白样品为基体进行加标回收试验,所得回收率在73.0%~121%之间,测定值的相对标准偏差(n=6)在0.90%~7.8%之间。
Abstract
HPLC was applied to the determination of 10 bis(triazinyl)aminostilbene fluorescent whitenings in mushrooms. For chromatographic separation,athena C18-WP chromatographic column (4.6 mm×200 mm,5 μm) was used as stationary phase,and mixtures of acetonitile and 0.02 mol·L-1 ammonium acetate solution with different mixing ratios were used as mobile phase in the gradient elution. Fluorescence detector was adopted in the determination with λex at 350 nm and λem at 430 nm. Linear relationships between values of peak area and mass concentration of the 10 fluorescent whitenings were kept in the same range of 2.0-100 μg·L-1,with detection limits (3S/N) in the range of 0.002-0.005 mg·kg-1. On the base of a blank sample,test for recovery was made by standard addition method,values of recovery found were in the range of 73.0%-121%,with RSD′s (n=6) in the range of 0.90%-7.8%.
中图分类号 O652.63 DOI 10.11973/lhjy-hx201511020
所属栏目 工作简报
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收稿日期 2015/2/27
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备注黄 薇(1963-),女,浙江宁波人,主任技师,主要从 事卫生理化检验工作。
引用该论文: HUANG Wei,ZHU Long-long,SUN Wen-fang,LIU Xiang-ping. HPLC Determination of 10 Fluorescent Whitenings in Mushrooms[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2015, 51(11): 1576~1579
黄 薇,朱龙龙,孙文芳,刘祥萍. 高效液相色谱法测定食用菌中10种荧光增白剂[J]. 理化检验-化学分册, 2015, 51(11): 1576~1579
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参考文献
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【2】陈立松,蒋丁国,傅武胜,等.纸制食品包装材料中荧光增白剂测定方法研究进展[J].卫生研究,2014,43(1):152-156.
【3】NY/T 1257-2006 食用菌中荧光物质的检测[S].
【4】马会娟,马强,白桦,等.洗涤用品中5种荧光增白剂的高效液相色谱法检测及质谱确证[J].分析试验室,2014,33(5):569-573.
【5】冼燕萍,郭新东,罗海英,等.固相萃取-高效液相色谱法分离测定洗涤用品中4种荧光增白剂[J].色谱,2013,31(2):162-169.
【6】焦艳娜,丁利,朱绍华,等.高效液相色谱荧光检测法测定食品接触材料塑料制品中荧光增白剂[J].色谱,2013,31(1):83-87.
【7】SHU Wei-chuan,DING Wang-hsien. Determination of fluorescent whitening agents in laundry detergents and surface waters by solid-phase extraction and ion-pair high performance liquid chromatography[J]. Journal of Chromatography A,2005,1088(1/2):218-233.
【8】吕春华,季鑫鹤,史颖珠,等.高效液相色谱法测定化妆品中5种荧光增白剂[J].理化检验-化学分册,2015,51(5):609-612.
【9】练习中,陈梅斯,郑丽斯,等.高效液相色谱法测定食品中荧光增白剂[J].现代食品科技,2011,27(10):1281-1284.
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