采用超高效液相色谱法同时测定食品中的安赛蜜、山梨酸、苯甲酸、脱氢乙酸和糖精钠的含量。不同食物样品经过前处理后,分析物经ACQUITY UPLC BEH C18色谱柱分离,以甲醇-含0.02 mol·L-1乙酸铵的乙酸溶液(pH 5.17)为流动相进行梯度洗脱,采用二极管阵列检测器,检测波长为230 nm。结果表明:5种食品添加剂在4 min内可以完全分离,线性范围均为0.10~50.0 mg·L-1,检出限在1.0~3.4 μg·L-1之间。加标回收率在90.1%~106%之间,测定值的相对标准偏差(n=7)在1.7%~4.9%之间。

UPLC was applied to the determination of acesulfame-k, sorbic acid, benzoic acid, dehydroacetic and sodium saccharin in food. After pretreatment of the samples, analytes were seperated on a ACQUITY UPLC BEH C18 column, using a mixture of methanol and 0.02 mol·L-1 ammonium acetate in acetic acid (pH 5.17) as the mobile phase for gradient elution. Diode array detector was adopted with the detective wavelength of 230 nm. Results showed that 5 additives could be well separated within 4 minutes, and the linear ranges of 5 additives were all 0.10-50.0 mg·L-1 with detection limits in the range of 1.0-3.4 μg·L-1. Recovery rates obtained by standard addition method were in the range of 90.1%-106%, and RSDs (n=7) were in the range of 1.7%-4.9%.