Preparation of Gold Nanoclusters and Its Application to Determination of H2O2 in Food
摘 要
以胰蛋白酶作为保护剂和还原剂,在胰蛋白酶和氯金酸混合溶液中合成了具有红色荧光的水溶性金纳米团簇(T-Au NCs)。考察了合成时间以及胰蛋白酶溶液的质量浓度对T-Au NCs荧光性能的影响,确立了最佳合成条件。荧光光谱表征说明T-Au NCs的最大激发和发射波长分别为500,640 nm,采用X射线光电子能谱表征了T-Au NCs中金的价态,并计算得到T-Au NCs的荧光量子产率为2.2%。基于过氧化氢对金纳米团簇的荧光猝灭作用,采用荧光光谱法测定食品中的过氧化氢残留量。过氧化氢的浓度的线性范围为0.050~1.00 mmol·L-1,检出限为7 μmol·L-1。对4种食品中过氧化氢残留量的测定结果与国家标准方法的测定结果一致。
Abstract
Water-soluble gold nanoclusters (T-Au NCs) with red fluorescence were synthesized with trypsin as stabilizing and reducing agent in the mixed solution of trypsin and chloroauric acid. The optimum synthesis conditions for T-Au NCs were defined by investigating the reaction time and the mass concentration of trypsin solution. Fluorescence spectroscopy characterization indicated the maximum excitation and emission wavelengths of the prepared T-Au NCs were 500 nm and 640 nm respectively. The valence state of gold in T-Au NCs was estimated by X-ray photoelectron spectroscopy. The fluorescence quantum yield of T-Au NCs was 2.2%. Based on the fluorescence quenching effect of H2O2 to T-Au NCs, H2O2 residues in the food were determined by fluorescence spectroscopy. The linear range of H2O2 was 0.050-1.00 mmol·L-1, with detection limit of 7 μmol·L-1. The determination results of H2O2 in 4 kinds of food were in consistent with those obtained by standard method.
中图分类号 O657.3 DOI 10.11973/lhjy-hx201605003
所属栏目 试验与研究
基金项目 江苏出入境检验检疫局科技计划项目(2013KJ33)
收稿日期 2015/4/10
修改稿日期
网络出版日期
作者单位点击查看
联系人作者江涛(np640706@163.com)
备注江 涛(1968-),男,江苏南通人,工程师,主要从事化学分析工作。
引用该论文: JIANG Tao,NI Peng,SHI Jin-hui,ZHANG Xiao-dong,NI Tian-chen,WANG Jin-juan,LI Yang,WU You-fan. Preparation of Gold Nanoclusters and Its Application to Determination of H2O2 in Food[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2016, 52(5): 506~509
江涛,倪鹏,施锦辉,张晓冬,倪天宸,王金娟,李阳,吴忧凡. 金纳米团簇荧光材料的制备及其应用于测定食品中的过氧化氢[J]. 理化检验-化学分册, 2016, 52(5): 506~509
共有人对该论文发表了看法,其中:
人认为该论文很差
人认为该论文较差
人认为该论文一般
人认为该论文较好
人认为该论文很好
参考文献
【1】潘勇军,谢洪泉,谭晓明,等.碘量滴定法测定过氧化氢溶液浓度的改进[J].理化检验-化学分册, 2003,39(7): 404-405.
【2】陈易晖,刘艳,周建立,等.高效液相色谱-紫外检测法测定食品中的过氧化氢[J].光谱实验室, 2009,26(2): 414-417.
【3】陈亚红,刘红梅,田丰收,等.酶催化分光光度法测定过氧化氢[J].理化检验-化学分册, 2009,45(4): 401-403.
【4】LI S, DONG S, NIENHAUS G U. Ultra-small fluorescent metal nanoclusters: synthesis and biological applications[J]. Nanotoday, 2011,6(4): 401-408.
【5】LIN C A J, LEE C H, YEH H I, et al. Fluorescent gold nanoclusters for biomedical applications, micro-and nanosystems in medicine, active implants[J]. Biosensors, 2009,25(8): 108-111.
【6】任娟,代雯乐,付欣,等.金团簇的荧光性质及其生物应用[J].化学通报, 2011,74(8): 683-687.
【7】FANG W, DONG Y H, FENG L, et al. Horseradish peroxidase functionalized fluorescent gold nanoclusters for hydrogen peroxide sensing[J]. Analytical Chemistry, 2011,83: 1193-1196.
【8】NEGISHI Y, NOBUSADA K, TSUKUDA T. Glutathione-protected gold clusters revisited: bridging the gap between gold(i)-thiolate complexes and thiolate-protected gold nanocrystals[J]. Journal of the American Chemical Society, 2005,127(14): 5261-5270.
【2】陈易晖,刘艳,周建立,等.高效液相色谱-紫外检测法测定食品中的过氧化氢[J].光谱实验室, 2009,26(2): 414-417.
【3】陈亚红,刘红梅,田丰收,等.酶催化分光光度法测定过氧化氢[J].理化检验-化学分册, 2009,45(4): 401-403.
【4】LI S, DONG S, NIENHAUS G U. Ultra-small fluorescent metal nanoclusters: synthesis and biological applications[J]. Nanotoday, 2011,6(4): 401-408.
【5】LIN C A J, LEE C H, YEH H I, et al. Fluorescent gold nanoclusters for biomedical applications, micro-and nanosystems in medicine, active implants[J]. Biosensors, 2009,25(8): 108-111.
【6】任娟,代雯乐,付欣,等.金团簇的荧光性质及其生物应用[J].化学通报, 2011,74(8): 683-687.
【7】FANG W, DONG Y H, FENG L, et al. Horseradish peroxidase functionalized fluorescent gold nanoclusters for hydrogen peroxide sensing[J]. Analytical Chemistry, 2011,83: 1193-1196.
【8】NEGISHI Y, NOBUSADA K, TSUKUDA T. Glutathione-protected gold clusters revisited: bridging the gap between gold(i)-thiolate complexes and thiolate-protected gold nanocrystals[J]. Journal of the American Chemical Society, 2005,127(14): 5261-5270.
相关信息