在pH 4.3的柠檬酸-磷酸氢二钠缓冲溶液中,以牛血红蛋白为催化剂,加速过氧化氢与过量的KI反应生成I3-,I3-再与碱性染料乙基紫结合生成离子缔合物颗粒,大大增强了体系的共振散射强度,据此建立了一种测定水中痕量过氧化氢的共振散射光谱法。在659 nm处,体系的共振散射强度增加值(ΔI)与过氧化氢的浓度在1.033×10-7~2.272×10-6mol·L-1范围内呈线性关系,检出限(3s/k)为1.64×10-8mol·L-1。方法用于测定雨水中的过氧化氢,测定值的相对标准偏差(n=5)小于5.0%,加标回收率在104%~105%之间。

In pH 4.3 citric acid-disodium hydrogen phosphate buffer solution, bovine hemoglobin as a catalyst could accelerate the reaction rate of hydrogen peroxide oxidizing the excess KI to I3-, and I3- combined with basic dye ethyl violet to form ion association particles, which enhanced the resonance light scattering intensity greatly. Base on this, a new resonance scattering spectrometry was proposed for the determination of trace hydrogen peroxide in water samples. The increased resonance light scattering intensity (ΔI) at 659 nm was linear to the concentration of hydrogen peroxide in the range of 1.033×10-7-2.272×10-6mol·L-1, with the detection limit (3s/k) of 1.64×10-8mol·L-1. The method was applied to the determination of hydrogen peroxide in rain water samples, giving RSDs (n=5) less than 5.0% and recovery rates obtained by standard addition method in the range of 104%-105%.