提出了微波辅助-顶空固相微萃取(MAE-HS-SPME)-气相色谱-质谱法测定不同产地烟草中挥发性成分。通过质谱标准谱图检索与色谱保留指数相结合的二维定性方法分析,以峰面积归一化法计算各组分的相对含量。结果表明:从我国湖南、贵州和云南省以及从津巴布韦等产地的烟草中分别定性检出24,28,27,24种挥发性成分,包括酯类、酮类、醇类、酸类、杂环、烷烃类等6大类化合物,以酯类和烷烃类为主。不同产地烟草所含化学成分的种类基本相同,但是其相对含量差异较大。MAE-HS-SPME是一种简便、快速、高效、绿色环保的前处理技术。

A method of GC-MS for the analysis of volatile components in tobaccos from different growing areas with MAE-HS-SPME was proposed. The compounds were identified by searching with standard mass spectra and by the retention indices. Values of relative contents of the individual components were calculated by normalization of peak areas. It was found that 24, 28, 27 and 24 compounds were identified in tobaccos grown in Hunan, Guizhou, Yunnan provinces of China and in Zimbabwe, respectively, including esters, ketones, alcohols, acids, heterocyclics and alkanes, giving priority to esters and alkanes. It was found that the groupings of the volatile components in tobaccos from different growing areas were basically the same, while their values of relative contents were quite different. The MAE-HS-SPME method proposed was proved to be a simpler, faster, more efficient and environment-friendly sample pretreatment procedure.