Determination of Nicotine in Simulated Saliva Dissolved from Bagged Smokeless Sucking Tobacco by UV-Vis Spectrophotometry
摘 要
袋装口含烟样品经自行设计的溶出装置使其中的烟碱溶入于人工唾液中,从所收集的人工唾液中分取试液1.00 mL,加入pH 4.6的柠檬酸-磷酸氢二钠缓冲溶液1.0 mL和饱和甲基橙溶液1.0 mL,混匀,生成黄色络合物。加入二氯甲烷4.0 mL,涡旋1 min,静置15 min,取下层萃取液,于波长420 nm处测量其吸光度。烟碱的质量浓度在1.00~150 mg·L-1范围内与吸光度呈线性关系,检出限(3s)为0.08 mg·L-1。按标准加入法进行回收试验,回收率在97.3%~103%之间,测定值的相对标准偏差(n=6)在0.86%~1.2%之间。
Abstract
By the self-made installation, the main constituents of the bagged smokeless sucking tobacco (BST), including nicotine were dissolved into simulated saliva, which was collected as described. An aliquot of 1.00 mL was taken from it for analysis, and 1.0 mL of citric acid-disodium hydrogen phospate buffer solution (pH 4.6) together with 1.0 mL of saturated methyl orange solution was added and mixed thoroughly to form a yellow colored complex with nicotine. 4.0 mL of dichloromethane was added to the solution, and the mixture was swirled for 1 min and stood for 15 min to have the nicotine-methylorange complex transferred into the organic phase. The lower organic phase was separated and its absorbance was measured at the wavelength of 420 nm. Linear relationship between values of absorbance and mass concentration of nicotine was found in the range of 1.00-150 mg·L-1 with detection limit (3s) of 0.08 mg·L-1. Test for recovery was made by standard addition method, giving results in the range of 97.3%-103%, with RSDs (n=6) in the range of 0.86%-1.2%.
中图分类号 O657.32 DOI 10.11973/lhjy-hx201704002
所属栏目 试验与研究
基金项目 中国烟草总公司科技重大专项[110201401017(XX-05)];云南中烟工业公司科技开发计划(2015CP07);袋装口含型无烟烟草制品研发(JSZX2014CP06)
收稿日期 2016/3/23
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联系人作者陈永宽(cyk1966@163.com)
备注袁大林(1979-),男,湖南新化人,工程师,主要从事烟草化学研究。
引用该论文: YUAN Da-lin,YANG Ji,TANG Jian-guo,YANG Liu,WU Jun,DUAN Yuan-xing,GONG Xiao-wei,CHEN Yong-kuan. Determination of Nicotine in Simulated Saliva Dissolved from Bagged Smokeless Sucking Tobacco by UV-Vis Spectrophotometry[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2017, 53(4): 377~380
袁大林,杨继,汤建国,杨柳,吴俊,段沅杏,巩效伟,陈永宽. 紫外-可见分光光度法测定袋装口含烟溶入人工唾液中的烟碱含量[J]. 理化检验-化学分册, 2017, 53(4): 377~380
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参考文献
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【8】WEST R. Tobacco control:present and future[J]. British Medical Bulletin, 2006,77/78(1):123-136.
【9】YC/T 246-2008 烟草及烟草制品 烟碱的测定 气相色谱法[S].
【10】YC/T 383-2010 烟草及烟草制品 烟碱、降烟碱、新烟碱、麦斯明和假木贼碱的测定 气相色谱-质谱联用法[S].
【11】张杰.口含型无烟气烟草制品中烟碱和烟草特有亚硝胺的释放行为研究[D].郑州:中国烟草总公司郑州烟草研究院, 2011.
【12】李维莉,马银海,段姚俊,等.紫外分光光度法测定烟草中的游离烟碱[J].安徽农业科学, 2012,40(6):3600-3601.
【2】BATES C, FAGERSTRÖM K, JARVIS M J, et al. European Union policy on smokeless tobacco: a statement in favour of evidence based regulation for public health[J]. Tobacco Control, 2003,12(4):360-367.
【3】BROADSTOCK M. Systematic review of the health effects of modified smokeless tobacco products[M]. New Zealand: Nzhta Report, 2007.
【4】FOULDS J, RAMSTROM L, BURKE M, et al. Effect of smokeless tobacco (snus) on smoking and public health in Sweden[J]. Tobacco Control, 2003,12(4):349-359.
【5】KLUS H, KUNZE M, KONIG S, et al. Smokeless tobacco-an overview[J]. Contribution to Tobacco Research, 2009,23(5):248-276.
【6】HOUEZEC J L, MCNEILL A, BRITTON J. Tobacco, nicotine and harm reduction[J]. Drug and Alcohol Review, 2011,30(2):119-123.
【7】International Agency for Research on Cancer (IARC). Monographs on the evaluation of carcinogenic risk to humans (Volume 89): smokeless tobacco and some tobacco-specific N-nitrosamines[M]. Lyon: World Health Organization, 2007.
【8】WEST R. Tobacco control:present and future[J]. British Medical Bulletin, 2006,77/78(1):123-136.
【9】YC/T 246-2008 烟草及烟草制品 烟碱的测定 气相色谱法[S].
【10】YC/T 383-2010 烟草及烟草制品 烟碱、降烟碱、新烟碱、麦斯明和假木贼碱的测定 气相色谱-质谱联用法[S].
【11】张杰.口含型无烟气烟草制品中烟碱和烟草特有亚硝胺的释放行为研究[D].郑州:中国烟草总公司郑州烟草研究院, 2011.
【12】李维莉,马银海,段姚俊,等.紫外分光光度法测定烟草中的游离烟碱[J].安徽农业科学, 2012,40(6):3600-3601.
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