采用固相萃取-高效液相色谱法同时测定复杂食品基质中的柠檬黄、日落黄、苋菜红、胭脂红、诱惑红、酸性红、赤藓红、亮蓝等8种合成色素的含量。样品经乙醇-乙腈-甲醇-氨水（3+3+3+1）混合液超声提取3次后，提取液采用ProElut PWA-2固相萃取柱净化，净化液在Agilent Eclipse XDB-C₁₈色谱柱上分离，以乙腈-0.02 mol·L^-1乙酸铵溶液为流动相进行梯度洗脱，采用二极管阵列检测器，检测波长为254 nm。8种合成色素的峰面积与其质量浓度在1.0~50.0 mg·L^-1内呈线性关系，检出限（3S/N）在0.25~0.80 μg·kg^-1之间。加标回收率为84.6%~99.0%，测定值的相对标准偏差（n=6）在1.4%~2.5%之间。

HPLC combined with solid phase extraction was applied to the simultaneous determination of 8 kinds of synthetic colorants, including tartrazine, sunset yellow, amaranth, carmine, allura red, azorubin, erythrosine and brilliant blue in complex food matrix. The sample was extracted ultrasonically with a mixture of ethanol-acetonitrile-methanol-ammonia (3+3+3+1) for 3 times. The extract was cleaned up by using a ProElut PWA-2 solid phase extraction column. The purified solution was seperated on an Agilent Eclipse XDB-C₁₈ column, with a mixture of acetonitrile and 0.02 mol·L^-1 ammonium acetate solution as the mobile phase for gradient elution. The analytes were detected by using diode array detector at the wavelength of 254 nm. Linear relationships were found between the peak areas and the mass concentrations of the 8 kinds of synthetic colorants in the range of 1.0-50.0 mg·L^-1, with detection limits in the range of 0.25-0.80 μg·kg^-1. Recovery rates obtained by standard addition method were in the range of 84.6%-99.0% and RSDs (n=6) were in the range of 1.4%-2.5%.