UV-Spectrophotometric Determination of Rutin in Medicine and Food Products with Separation by SPE Using Ionic Liquid Loaded β-Cyclodextrin-Cross-Linked Polymer as Solid Extractant
摘 要
以自制的咪唑类离子液体-β-环糊精聚合物(IL-β-CDCP)作为萃取剂,对药品和食品中芦丁的固相萃取(SPE)分离条件进行了优化,并用紫外分光光度法测定样品中芦丁的含量。结果表明:在含芦丁的pH 4.0乙酸盐缓冲溶液40 mL中加入IL-β-CDCP 0.1 g,于15.0℃水浴中振荡10.0 min后,可使92.0%的芦丁吸附在IL-β-CDCP上。离心分离后,吸附了芦丁的IL-β-CDCP留在下层。将其分出并在其中加无水乙醇4.0 mL,于35.0℃水浴振荡10.0 min,可使芦丁从IL-β-CDCP洗脱,溶入于乙醇中,脱附率达94.0%,于360 nm处测量洗脱液的吸光度。芦丁的质量浓度在0.06~7.0 mg·L-1内与其吸光度呈线性关系,检出限(3s/k)为8.1 μg·L-1。测定值的相对标准偏差(n=6)为0.38%~1.7%。
Abstract
Self-prepared cross-linked polymer formed between imidazole ionic liquid and β-cyclodextrin (IL-β-CDCP) was used as solid extractant in the SPE of rutin from samples of medicine and food, and optimum conditions for the separation were studied. The analyte (rutin) was then determined by UV-spectrophotometry. As shown by the results, when 0.1 g of IL-β-CDCP was added to 40 mL of acetate buffer solution (pH 4.0) containing rutin, and the mixture was shaked for 10.0 min in a water bath heated at 15.0℃, 92.0% of the rutin was found adsorbed on IL-β-CDCP. After centrifugation, and removing the super liquid phase, absolute alcohol (4.0 mL) was added to the IL-β-CDCP adsorbed with rutin settled in the lower phase, and the mixture was shaked for 10.0 min at 35.0℃ in a water bath, during which 94.0% of the adsorbed rutin was eluted from IL-β-CDCP and dissolved in alcohol. The absorbance of the alcohol solution was measured at 360 nm. Linear relationship between values of absorbance and mass concentration of rutin was found in the range of 0.06 to 7.0 mg·L-1, with detection limit (3s/k) of 8.1 μg·L-1. Values of RSD's (n=6) found were range from 0.38% to 1.7%.
中图分类号 O657.32 DOI 10.11973/lhjy-hx201708001
所属栏目 试验与研究
基金项目 南通市卫生局项目(WQ2014070)
收稿日期 2016/11/15
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备注平文卉(1988-),女,江苏高邮人,硕士研究生,主要从事理化检验研究工作
引用该论文: PING Wenhui,YANG Juan,SHI Yukun. UV-Spectrophotometric Determination of Rutin in Medicine and Food Products with Separation by SPE Using Ionic Liquid Loaded β-Cyclodextrin-Cross-Linked Polymer as Solid Extractant[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2017, 53(8): 869~873
平文卉,杨娟,史玉坤. 咪唑类离子液体-β-环糊精聚合物固相萃取-紫外分光光度法测定药品和食品中芦丁[J]. 理化检验-化学分册, 2017, 53(8): 869~873
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【4】SUN Y, LI W, WANG J, et al. Determination of rutin in cigarette tobacco, filters, mainstream smoke and burned ash of different branded cigarettes by high performance liquid chromatography[J]. Molecules, 2012,17(4):3751-3760.
【5】YIN H, ZHOU Y, CUI L, et al. Sensitive voltammetric determination of rutin in pharmaceuticals, human serum, and traditional Chinese medicines using a glassy carbon electrode coated with graphene nanosheets, chitosan, and a poly (amido amine) dendrimer[J]. Microchimica Acta, 2011,173(3):337-345.
【6】LI X, ANGST M S, CLARK J D. Opioid-induced hyperalgesia and incisional pain[J]. Anesthesia & Analgesia, 2001,93(1):204-209.
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