气相色谱-质谱法测定胶基型嚼烟中烟碱的含量
GC-MS Determination of Nicotine in Gum Chewing Tobacco
摘 要
取（1.42±0.01）g的试样5粒，溶解在5 mol·L-1氢氧化钠溶液8 mL和含内标的正己烷40 mL中，磁力搅拌110 min，静置20 min。取上层萃取液2 mL，用无水硫酸钠干燥，过0.22 μm有机相滤膜。所得溶液采用DB-5MS色谱柱进行气相色谱分离。质谱分析采用选择离子监测模式。烟碱的线性范围为4.00~200 mg·L-1，检出限（3s）为0.19 mg·L-1。按标准加入法进行回收试验，回收率在91.0%~96.1%之间，测定值的相对标准偏差（n=6）均小于3.0%。
胶基型嚼烟
烟碱
GC-MS
gum chewing tobacco
nicotine
Abstract
5 pellets of the sample of (1.42±0.01) g were dissolved with 8 mL of 5 mol·L-1 sodium hydroxide solution and 40 mL of hexane containing internal standard substance. The solution was magnetically stirred for 110 min, and stood for 20 min. An aliquot of 2 mL of the extract from the upper layer was dried with anhydrous sodium sulfate, and filtered through organic filter membrane of 0.22 μm. The sample solution was separated by GC using DB-5MS chromatographic column. SIM was adopted in MS analysis. Linearity range of nicotine was 4.00-200 mg·L-1, with detection limit (3s) of 0.19 mg·L-1. Test for recovery was made by standard addition method, giving results in the range of 91.0%-96.1%, with RSDs (n=6) all less than 3.0%.
中图分类号 O657.63 DOI 10.11973/lhjy-hx201710002
所属栏目 试验与研究
基金项目 中国烟草总公司科技重大专项[110201401017（XX-05）]；云南中烟工业公司科技开发计划（2015CP07）；云南中烟工业有限责任公司重点项目（2016XY02）
收稿日期 2016/10/23
修改稿日期
网络出版日期
作者单位点击查看
联系人作者汤建国(jgtang@163.com)
备注杨继(1980-),女,云南昆明人,工程师,硕士,主要从事烟草化学研究
引用该论文: YANG Ji,YUAN Dalin,HONG Liu,DUAN Yuanxing,TIAN Yongfeng,YANG Liu,ZHAO Wei,TANG Jianguo,CHEN Yongkuan,MIAO Mingming. GC-MS Determination of Nicotine in Gum Chewing Tobacco[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2017, 53(10): 1124～1128
杨继,袁大林,洪鎏,段沅杏,田永峰,杨柳,赵伟,汤建国,陈永宽,缪明明. 气相色谱-质谱法测定胶基型嚼烟中烟碱的含量[J]. 理化检验－化学分册, 2017, 53(10): 1124～1128
共有人对该论文发表了看法，其中：
人认为该论文很差
人认为该论文较差
人认为该论文一般
人认为该论文较好
人认为该论文很好
参考文献
【1】STEPANOV I, JENSEN J, HATSUKAMI D, et al. New and traditional smokeless tobacco:Comparison of toxicant and carcinogen levels[J]. Nicotine & Tobacco Research, 2008,10(12):1773-1782.
【2】BATES C, FAGERSTROM K, JARVIS M J, et al. European Union policy on smokeless tobacco:A statement in favour of evidence based regulation for public health[J]. Tobacco Control, 2003,12(4):360-367.
【3】BROADSTOCK M. Systematic review of the health effects of modified smokeless tobacco products[M]. New Zealand:Nzhta Report, 2007.
【4】FOULDS J, RAMSTROM L, BURKE M, et al. Effect of smokeless tobacco (snus) on smoking and public health in Sweden[J]. Tobacco Control, 2003,12(4):349-359.
【5】KLUS H, KUNZE M, KONIG S, et al. Smokeless tobacco-an overview[J]. Beitrage zur Tabakforschung International/Contributions to Tobacco Research, 2009,23(5):248-276.
【6】LE HOUEZEC J, MCNEILL A, BRITTON J. Tobacco, nicotine and harm reduction[J]. Drug and Alcohol Review, 2011,30(2):119-123.
【7】International Agency for Research on Cancer (IARC). Monographs on the evaluation of carcinogenic risk to humans (volume 89):Smokeless tobacco and some tobacco-specific N-nitrosamines[M]. Lyon:World Health Organization, 2007.
【8】红塔烟草(集团)有限责任公司.一种烟草口香糖及其制备方法:103719525A[P]. 2014-04-16.
【9】中国烟草总公司广东省公司.一种全烟草组分的胶基型口香烟及其制备方法:105054283A[P]. 2015-11-18.
【10】奥克诺有限公司.烟草口香糖制剂:103732075A[P]. 2014-04-16.
【11】费尔廷制药公司.释放烟草生物碱的口香糖:1997350A[P]. 2007-07-11.
【12】中国烟草总公司郑州烟草研究院.板状口嚼式烟糖:201171342Y[P].2008-12-31.
【13】HATSUKAMI D K, SEVERSON H H. Oral spit tobacco:Addiction, prevention and treatment[J]. Nicotine & Tobacco Research, 1999,1(1):21-24.
【14】DJORDJEVIC M V, HOFFMANN D, GLYNN T, et al. US commercial brands of moist snuff, 1994-assessment of nicotine, moisture, and pH[J]. Tobacco Control,1995,4(1):62-66.
【15】殷全玉,杨铁钊,郭宝银,等.紫外分光光度法测定烟草中的游离烟碱[J].中国烟草科学, 2008,29(6):20-22.
【16】黄晖,黄海东,李燕垣.气相色谱法测定卷烟烟气总粒相物中的烟碱[J].烟草科技, 1997(5):23-24.
【17】谢笑天,郑萍,朱伟明,等.高效液相色谱法测定卷烟烟气总粒相物中的烟碱[J].分析化学, 2000,28(9):1085-1087.
【18】阎瑾,鲍峰伟,牛丽娜,等.气相色谱-质谱法测定主流烟气中的植物碱[J].理化检验-化学分册, 2015,51(9):1252-1255.
【19】张杰,李鹏,宗永立,等.国外口含型无烟气烟草制品总烟碱、游离烟碱和烟草特有亚硝胺含量分析[J].烟草科技, 2011(10):44-48.
【20】ZANDER A, FINDLAY P, RENNER T, et al. Analysis of nicotine and its oxidation products in nicotine chewing gum by a molecularly imprinted solid-phase extraction[J]. Analytical Chemistry, 1998,70(15):3304-3314.
【21】ZUO Y G, ZHANG L L, WU J P, et al. Ultrasonic extraction and capillary gas chromatography determination of nicotine in pharmaceutical formulations[J]. Analytica Chimica Acta, 2004,526(1):35-39.
【22】SELLERGREN B, ZANDER A, RENNER T, et al. Rapid method for analysis of nicotine and nicotine-related substances in chewing gum formulations[J]. Journal of Chromatography A, 1998,829(1/2):143-152.
【2】BATES C, FAGERSTROM K, JARVIS M J, et al. European Union policy on smokeless tobacco:A statement in favour of evidence based regulation for public health[J]. Tobacco Control, 2003,12(4):360-367.
【3】BROADSTOCK M. Systematic review of the health effects of modified smokeless tobacco products[M]. New Zealand:Nzhta Report, 2007.
【4】FOULDS J, RAMSTROM L, BURKE M, et al. Effect of smokeless tobacco (snus) on smoking and public health in Sweden[J]. Tobacco Control, 2003,12(4):349-359.
【5】KLUS H, KUNZE M, KONIG S, et al. Smokeless tobacco-an overview[J]. Beitrage zur Tabakforschung International/Contributions to Tobacco Research, 2009,23(5):248-276.
【6】LE HOUEZEC J, MCNEILL A, BRITTON J. Tobacco, nicotine and harm reduction[J]. Drug and Alcohol Review, 2011,30(2):119-123.
【7】International Agency for Research on Cancer (IARC). Monographs on the evaluation of carcinogenic risk to humans (volume 89):Smokeless tobacco and some tobacco-specific N-nitrosamines[M]. Lyon:World Health Organization, 2007.
【8】红塔烟草(集团)有限责任公司.一种烟草口香糖及其制备方法:103719525A[P]. 2014-04-16.
【9】中国烟草总公司广东省公司.一种全烟草组分的胶基型口香烟及其制备方法:105054283A[P]. 2015-11-18.
【10】奥克诺有限公司.烟草口香糖制剂:103732075A[P]. 2014-04-16.
【11】费尔廷制药公司.释放烟草生物碱的口香糖:1997350A[P]. 2007-07-11.
【12】中国烟草总公司郑州烟草研究院.板状口嚼式烟糖:201171342Y[P].2008-12-31.
【13】HATSUKAMI D K, SEVERSON H H. Oral spit tobacco:Addiction, prevention and treatment[J]. Nicotine & Tobacco Research, 1999,1(1):21-24.
【14】DJORDJEVIC M V, HOFFMANN D, GLYNN T, et al. US commercial brands of moist snuff, 1994-assessment of nicotine, moisture, and pH[J]. Tobacco Control,1995,4(1):62-66.
【15】殷全玉,杨铁钊,郭宝银,等.紫外分光光度法测定烟草中的游离烟碱[J].中国烟草科学, 2008,29(6):20-22.
【16】黄晖,黄海东,李燕垣.气相色谱法测定卷烟烟气总粒相物中的烟碱[J].烟草科技, 1997(5):23-24.
【17】谢笑天,郑萍,朱伟明,等.高效液相色谱法测定卷烟烟气总粒相物中的烟碱[J].分析化学, 2000,28(9):1085-1087.
【18】阎瑾,鲍峰伟,牛丽娜,等.气相色谱-质谱法测定主流烟气中的植物碱[J].理化检验-化学分册, 2015,51(9):1252-1255.
【19】张杰,李鹏,宗永立,等.国外口含型无烟气烟草制品总烟碱、游离烟碱和烟草特有亚硝胺含量分析[J].烟草科技, 2011(10):44-48.
【20】ZANDER A, FINDLAY P, RENNER T, et al. Analysis of nicotine and its oxidation products in nicotine chewing gum by a molecularly imprinted solid-phase extraction[J]. Analytical Chemistry, 1998,70(15):3304-3314.
【21】ZUO Y G, ZHANG L L, WU J P, et al. Ultrasonic extraction and capillary gas chromatography determination of nicotine in pharmaceutical formulations[J]. Analytica Chimica Acta, 2004,526(1):35-39.
【22】SELLERGREN B, ZANDER A, RENNER T, et al. Rapid method for analysis of nicotine and nicotine-related substances in chewing gum formulations[J]. Journal of Chromatography A, 1998,829(1/2):143-152.
相关信息
