Menaquinone-7原料药在避光条件下经无水乙醇超声溶解后，采用Agilent Eclipse plus C₁₈色谱柱（2.1 mm×50 mm，1.8 μm）分离主成分及有关物质，以甲醇-水（98+2）混合液为流动相，分别在波长246，270 nm处测定主成分及有关物质。Menaquinone-7的线性范围在0.005~0.400 g·L^-1内，检出限（3S/N）为0.1 mg·L^-1。加标回收率在98.8%~100%之间，测定值的相对标准偏差（n=6）小于1.0%。测得两批Menaquinone-7原料药中主成分的质量分数分别为98.0%，98.2%，单个杂质质量分数小于0.5%，杂质总质量分数小于1.0%。

Raw material drug of Menaquinone-7 was dissolved ultrasonically with ethanol in the dark, and then the principal compound and related substances in the sample were separated on an Agilent Eclipse plus C₁₈ chromatographic column (2.1 mm×50 mm, 1.8 μm). The mobile phase was methanol-water (98+2) solution, and the detection wavelengths were 246, 270 nm for the determination of principal compound and related substances, respectively. The linearity range of Menaquinone-7 was 0.005-0.400 g·L^-1, with detection limit (3S/N) of 0.1 mg·L^-1. Recovery rates obtained by standard addition method were in the range of 98.8%-100% and RSDs (n=6) were less than 1.0%. The contents of the principal compound in 2 batches of Menaquinone-7 raw material drug were 98.0% and 98.2%. The mass fraction of single impurity was less than 0.5%, and the mass fraction of total impurities was less than 1.0%.