GC Determination of 5 Bioamines in Bacon with Solid Phase Extraction
摘 要
采用固相萃取-气相色谱法测定腌肉中酪胺、组胺、精胺、亚精胺和β-苯乙胺等5种生物胺的含量。腌肉样品用50 g·L-1三氯乙酸溶液提取,强阳离子交换固相萃取柱净化。用Agilent DB-1701色谱柱(30 m×0.25 mm,0.25 μm)分离,氮磷检测器检测。5种生物胺的质量浓度均在1.0~50.0 mg·L-1内与其对应的峰面积呈线性关系,方法的检出限(3S/N)为0.12~0.32 mg·kg-1。以空白样品为基体进行加标回收试验,所得回收率为78.0%~96.8%,测定值的相对标准偏差(n=6)为2.2%~7.1%。
Abstract
GC was applied to the determination of 5 bioamines, i.e. tyramine, histamine, spermine, spermidine and phenylethylamine in bacon with solid phase extraction. The bacon sample was extracted with 50 g·L-1 trichloroacetic acid solution and purified by SCX solid phase extraction column. The analytes were separated on Agilent DB-1701 chromatographic column (30 m×0.25 mm, 0.25 μm) and detected by NPD. Linear relationships between values of peak area and mass concentration of the 5 bioamines were all kept in the same range of 1.0-50.0 mg·L-1, with detection limits (3S/N)in the range of 0.12-0.32 mg·kg-1. On the base of blank sample, test for recovery was made by standard addition method. Values of recovery found were in the range of 78.0%-96.8%, with RSD's (n=6) in the range of 2.2%-7.1%.
中图分类号 O657.7 DOI 10.11973/lhjy-hx201805004
所属栏目 工作简报
基金项目 江苏高校品牌专业建设工程资助项目(PPZY2015B180)
收稿日期 2017/6/19
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备注王芳,讲师,硕士,研究方向为工业催化,393231106@qq.com
引用该论文: WANG Fang,LUO Min,TAO Wenbo,XU Lin. GC Determination of 5 Bioamines in Bacon with Solid Phase Extraction[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2018, 54(5): 512~515
王芳,罗敏,陶文波,徐林. 固相萃取-气相色谱法测定腌肉中5种生物胺[J]. 理化检验-化学分册, 2018, 54(5): 512~515
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参考文献
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【2】REDRUELLO B, LADERO V, DEL RIO B, et al. A UHPLC method for the simultaneous analysis of biogenic amines, amino acids and ammonium ions in beer[J]. Food Chemistry, 2017,217:117-124.
【3】温永柱,范文来,徐岩.GC-MS法定性白酒中的多种生物胺[J].酿酒, 2013(1):38-41.
【4】王树庆,范维江,李成凤.肉及肉制品中的生物胺[J].食品研究与开发, 2016(24):203-206.
【5】GB 5009.208-2016食品安全国家标准食品中生物胺的测定[S].
【6】乔成栋,宋平顺,严祥,等.5种生物胺的毛细管胶束电动色谱分离[J].分析化学, 2007,35(1):95-98.
【7】吴云辉,周爽,徐敦明.非衍生化液相色谱-串联质谱法测定动物源性食品中8种生物胺[J].色谱, 2013,31(2):111-116.
【8】马明,沈劼,张峥,等.气固顶空-气相色谱法测定纺织品中N,N-二甲基甲酰胺与N,N-二甲基乙酰胺[J].理化检验-化学分册, 2015,51(1):18-21.
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