胶体金免疫层析法检测水产品中15种喹诺酮类药物
Detection of 15 Quinolones in Aquatic Products by Colloidal Gold Immunochromatography Assay
摘 要
采用胶体金免疫层析法检测水产品中恶喹酸、恩诺沙星、环丙沙星、二氟沙星、氟甲喹、沙拉沙星、达氟沙星、诺氟沙星、培氟沙星、依诺沙星、那氟沙星、氧氟沙星、奥比沙星、洛美沙星和麻保沙星等15种喹诺酮类药物。水产品样品用0.1%（体积分数）甲酸乙腈溶液提取，正己烷脱脂，胶体金试纸条检测，检测时间只需3~5 min。15种喹诺酮类药物的检出限在0.3~5 μg·kg-1之间。对400批次样品进行检测，发现9批次阳性样品，经超高效液相色谱-串联质谱法（UHPLC-MS/MS）验证，检测结果一致。
水产品
喹诺酮类药物
colloidal gold immunochromatography assay
aquatic product
quinolone
Abstract
Colloidal gold immunochromatography assay was applied to the detection of 15 quinolones, i.e. Oxolinic acid, Enrofloxacin, Ciprofloxacin, Difloxacin, Flumequine, Sarafloxacin, Danofloxacin, Norfloxacin, Pefloxacin, Enoxacin, Nadifloxacin, Ofloxacin, Orbifloxacin, Lomefloxacin and Marbofloxacin in aquatic products. The sample of aquatic product was extracted with 0.1%(φ) formic acid acetonitrile solution, and n-hexane was added for defatting. Finally the solution was detected with colloidal gold test strip, and detection time was only 3-5 minutes. Detection limits of 15 quinolones were in the range of 0.3-5 μg·kg-1. 400 batches of the samples were detected, and 9 batches of positive samples were found. The result was verifyied by UHPLC-MS/MS.
中图分类号 O65 DOI 10.11973/lhjy-hx201805022
所属栏目 专题报道(食品分析)
基金项目 浙江省科技计划项目（2016F30022，2017F50018）
收稿日期 2017/9/8
修改稿日期
网络出版日期
作者单位点击查看
备注宗婧婧,硕士研究生,研究方向为水产品的质量与安全
引用该论文: ZONG Jingjing,ZHANG Xiaojun,YAN Zhongyong,LU Yibo,LI Tingting,GAO Xuehui,ZHANG Xun. Detection of 15 Quinolones in Aquatic Products by Colloidal Gold Immunochromatography Assay[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2018, 54(5): 591～595
宗婧婧,张小军,严忠雍,卢义博,李停停,高学慧,张勋. 胶体金免疫层析法检测水产品中15种喹诺酮类药物[J]. 理化检验－化学分册, 2018, 54(5): 591～595
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参考文献
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【25】李佩佩,郭远明,陈雪昌,等.色谱法检测动物源食品中喹诺酮类药物残留研究进展[J].食品科学, 2013,34(3):303-307.
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【2】房文红,于慧娟,蔡友琼,等.恩诺沙星及其代谢物环丙沙星在欧洲鳗鲡体内的代谢动力学[J].中国水产科学, 2007(4):622-629.
【3】丁国华,丁磊如,刘霞.氟喹诺酮类药的神经毒性作用[J].中国医院药学杂志, 1995(8):380-381.
【4】CABELLO F C. Heavy use of prophylactic antibiotics in aquaculture:A growing problem for human and animal health and for the environment[J]. Environmental Microbiology, 2006,8(7):1137-1144.
【5】刘春娥,刘峰.水产品氟喹诺酮类药物残留检测研究进展[J].安徽农业科学, 2010,38(3):1116-1118.
【6】ORGANIZATION W H. Use of quinolones in food animals and potential impact on human health:Report and proceedings of a WHO meeting, Geneva, Switzerland 2-5 June 1998[M]. Geneva:World Health Organization, 1998.
【7】DESMET G. Commission of the European communities[J]. Journal of Hospice & Palliative Nursing, 1989,85(1):81-91.
【8】NAGAYAMA T. Positive list system for agricultural chemical residues in foods[J]. Journal of Pesticide Science, 2005,30(4):418-425.
【9】农业部235号公告动物性食品中兽药最高残留限量[S].
【10】HUET A C, CHARLIER C, TITTLEMIER S A, et al. Simultaneous determination of (fluoro)quinolone antibiotics in kidney, marine products, eggs, and muscle by enzyme-linked immunosorbent assay (ELISA)[J]. Journal of Agricultural and Food Chemistry, 2006,54(8):2822-2827.
【11】王强,王旭峰,杨金兰,等.直接竞争ELISA法快速测定水产品中6种氟喹诺酮类药物[J].食品工业科技, 2014(8):61-65.
【12】李存,江海洋,吴银良,等.高效液相色谱-荧光-紫外法测定动物肌肉组织中多类药物残留[J].分析化学, 2009,37(8):1102-1106.
【13】李佐卿,倪梅林,章再婷,等.高效液相色谱法检测水产品中喹诺酮类药物残留[J].现代科学仪器, 2006(3):70-71.
【14】PECORELLI I, GALARINI R, BIBI R, et al. Simultaneous determination of 13 quinolones from feeds using accelerated solvent extraction and liquid chromatography[J]. Analytica Chimica Acta, 2003,483(1):81-89.
【15】杨方,庞国芳,刘正才,等.液相色谱-串联质谱法检测水产品中15种喹诺酮类药物残留量[J].分析试验室, 2008(12):27-33.
【16】岳振峰,林秀云,唐少冰,等.高效液相色谱-串联质谱法测定动物组织中的16种喹诺酮类药物残留[J].色谱, 2007(4):491-495.
【17】LOMBARDO-AGVÍ M, GARCÍA-CAMPAÑA A M, GÁMIZ-GRACIA L, et al. Determination of quinolones of veterinary use in bee products by ultra-high performance liquid chromatography-tandem mass spectrometry using a QuEChERS extraction procedure[J]. Talanta, 2012,93(20):193-199.
【18】黄晓蓉,郑晶,李寿崧,等.鳗鱼及其制品中喹诺酮类药物残留的微生物快速检测方法研究[J].淡水渔业, 2005(4):3-6.
【19】薛晓,荣霞,叶自霞,等.胶体金免疫层析法在畜禽产品兽药残留检测中的应用[J].动物医学进展, 2013,34(12):179-182.
【20】陈一鸣.胶体金免疫层析法在畜禽产品兽药残留检测中的应用[J].中国畜牧兽医文摘, 2017,33(5):215-215.
【21】曹德康,苏建忠,初晨,等.胶体金免疫层析法同时检测肉类食品中八种兽药残留物[J].解放军预防医学杂志, 2017,35(3):213-217.
【22】吴刚,姜瞻梅,霍贵成,等.胶体金免疫层析技术在食品检测中的应用[J].食品工业科技, 2007(12):216-218.
【23】LIU L, LUO L, SURYOPRABOWO S, et al. Development of an immunochromatographic strip test for rapid detection of ciprofloxacin in milk samples[J]. Sensors, 2014,14(9):16785-16798.
【24】ZHU Y, LI L, WANG Z H, et al. Development of an immunochromatography strip for the rapid detection of 12 fluoroquinolones in chicken muscle and liver[J]. Journal of Agricultural and Food Chemistry, 2008,56(14):5469-5474.
【25】李佩佩,郭远明,陈雪昌,等.色谱法检测动物源食品中喹诺酮类药物残留研究进展[J].食品科学, 2013,34(3):303-307.
【26】万宇平,刘清珺,贾芳芳,等.一种快速检测氟喹诺酮类药物的胶体金试纸卡的研制[J].中国乳品工业, 2013,41(8):20-23.
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