Determination of 8-Hydroxy-2'-deoxyguanosine Using Glassy Carbon Electrode Modified with Metal Organic Framework and Ordered Mesoporous Carbon
摘 要
通过水热法制备了金属有机框架材料(ZIF-8),采用ZIF-8和有序介孔碳(OMC)修饰玻碳电极(GCE),采用循环伏安法和交流阻抗法对此修饰电极(ZIF-8/OMC/GCE)的电化学性能进行了研究。结果表明,经过修饰可增大裸玻碳电极的有效表面积、改善电极的电催化活性。利用差分脉冲伏安法研究了8-羟基脱氧鸟苷在ZIF-8/OMC/GCE上的电化学特性。结果表明,此修饰电极的电流响应值与8-羟基脱氧鸟苷的浓度在0.35~350 μmol·L-1内呈线性关系,检出限(3S/N)为0.22 μmol·L-1。采用修饰电极测定人尿样中的8-羟基脱氧鸟苷,加标回收率在90.1%~108%之间。
Abstract
A metal organic framework material (ZIF-8) was prepared by a hydrothermal method. The glassy carbon electrode (GCE) was modified by ZIF-8 and ordered mesoporous carbon (OMC). Electrochemical behavior of the modified electrode (ZIF-8/OMC/GCE) was studied by cyclic voltammetry and alternating current impedance, and as shown by the results, the effective surface area of the modified electrode was increased significantly as compared with the bare GCE, thus leading to improvement of electrocatalytic activity of the modified electrode. Electrochemical characteristics of 8-hydroxy-2'-deoxyguanosine (8-OHdG) at ZIF-8/OMC/GCE were studied by differential pulse voltammetry, and it was found that linear relationship was obtained between values of current response and concentration of 8-OHdG in the range of 0.35-350 μmol·L-1, with the detection limit (3S/N) of 0.22 μmol·L-1. The modified electrode was used to determine 8-OHdG in human urine samples, giving values of recovery obtained by standard addition method in the range of 90.1%-108%.
中图分类号 O657.1 DOI 10.11973/lhjy-hx201806003
所属栏目 试验与研究
基金项目 国家自然科学基金面上项目(81373047;81673229)
收稿日期 2017/6/14
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备注高牧丛,硕士研究生,研究方向为电化学传感器
引用该论文: GAO Mucong,ZHANG Pan,ZHOU Qiang,RONG Shengzhong,CHANG Dong,PAN Hongzhi. Determination of 8-Hydroxy-2'-deoxyguanosine Using Glassy Carbon Electrode Modified with Metal Organic Framework and Ordered Mesoporous Carbon[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2018, 54(6): 634~639
高牧丛,张盼,周强,荣胜忠,常东,潘洪志. 金属有机框架-有序介孔碳修饰的玻碳电极用于测定8-羟基脱氧鸟苷[J]. 理化检验-化学分册, 2018, 54(6): 634~639
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【3】ⅡDA T, FURUTA A, NISHIOKA K, et al. Expression of 8-oxoguanosine DNA glycosylase is reduced and associate with neurofibrillary tangles in Alzheimer, sdisease brain[J]. Acta Neuropathologica, 2002,103(1):20-25.
【4】蔡凌霜,曾昭睿,吴采樱.DNA氧化损伤物及其检测方法[J].分析测试学报, 2001,20(5):88-93.
【5】SANTELLA R M. Immunological methods for detection of carcinogen-DNA damage in humans[J]. Cancer Epidemiology Biomarkers & Prevention, 1999,8(9):733-739.
【6】TAMAE K, KAWAI K, YAMASAKI S, et al. Effect of age, smoking and other lifestyle factors on urinary 7-methylguanine and 8-hydroxydeoxyguanosine[J]. Cancer Science, 2009,100(4):715-721.
【7】张霞,苏本利,王赞峰,等.尿8-羟基脱氧鸟苷水平与糖尿病肾病的相关性[J].中国糖尿病杂志, 2006,14(1):41-42.
【8】HAN Y Y, DONOVAN M, SUNG F C. Increased urinary 8-hydroxy-2'-deoxy-guanosine excretion in long-distance bus drivers in Taiwan[J]. Chemosphere, 2010,79(9):942-948.
【9】梅素容,蔡凌霜,姚庆红,等.毛细管电泳-柱末安培检测癌症病人尿中8-OHdG[J].高等学校化学学报, 2003,24(11):1987-1989.
【10】GUTIÉRREZ A, GUTIÉRREZ S, GARCÍA G, et al. Determination of 8-hydroxy-2'-deoxyguanosine using electrodes modified with a dispersion of canbon nanotubes in polyethyenimine[J]. Electroanalysis, 2011,23(5):1221-1228.
【11】潘洪志,常东,那立欣,等.32P后标记法检测DNA中8-羟基脱氧鸟苷含量[J].中国卫生检验杂志, 2004,14(1):19-20.
【12】ZHU L, TIAN C Y, ZHU D X, et al. Ordered mesoporous carbon paste electrodes for electrochemical sensing and biosensing[J]. Electroanalysis, 2008,20(10):1128-1134.
【13】MA S, ZHOU H. Gas storage in porous metal-organic frameworks for clean energy applications[J]. Chemical Communications, 2010,46(1):44-53.
【14】DHAKSHINAMOORTHY A, GARCIA H. Catalysis by metal nanoparticles embedded on metal-organic frameworks[J]. Chemical Society Reviews, 2012,41(15):5262-5284.
【15】LING P, LEI J, ZHANG L, et al. Porphyrin-encapsulated metal-organics frameworks as mimetic catalysts for electrochemical DNA sensing via allosteric switch of hairpin DNA[J]. Analytical Chemistry, 2015,87(7):3957-3963.
【16】刘明明,吕文苗,史秀峰,等.不同方法合成的沸石咪唑酯骨架结构材料(ZIF-8)的表征和催化性能[J].无机化学学报, 2014,30(3):579-584.
【17】李启隆.电分析化学[M].北京:北京师范大学出版社, 1995:240-245.
【18】WANG L J, SHAO H, XU G, et al. Determination of 8-hydroxy2-deoxy-guanosine in human urine by high performance liquid chromatography[J]. Chinese Journal of Health Laboratory Technology, 2008,18(6):1030-1032.
【19】KVASNICOVÁ V, SAMCOVÁ E, JURSOVÁ A, et al. Determination of 8-hydroxy-2'-deoxyguanosine in untreated urine by capillary electrophoresis with UV detection[J]. Journal of Chromatography A, 2003,985(1/2):513-517.
【20】GUTIÉRREZ A, GUTIERREZ F A, EGUÍLAZ M, et al. Electrochemical sensing of guanine, adenine and 8-hydroxy-2-deoxyguanosine at glassy carbon modified with single-walled carbon nanotubes covalently functionalized with lysine[J]. RSC Advances, 2016,6(16):13469-13477.
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