采用高效液相色谱法测定聚氨酯胶黏剂施工后2种二异氰酸酯类化合物（2，6-甲苯二异氰酸酯和六亚甲基二异氰酸酯）的释放量。聚氨酯胶黏剂样品涂覆于玻璃板上，置于气候箱中，用含1-（2-甲氧基苯基）哌嗪的玻璃纤维滤膜采样，取出滤膜放于瓶中，加入0.2 mol·L^-1 1-（2-甲氧基苯基）哌嗪甲苯溶液、乙酸乙酯和乙酸酐。采用Hypersil ODS色谱柱为分离柱，以乙腈与pH 6.0的乙酸-乙酸钠缓冲溶液以体积比33：67组成的混合液为流动相，在检测波长242 nm处进行测定。2，6-甲苯二异氰酸酯和六亚甲基二异氰酸酯的质量浓度均在20.0 mg·L^-1以内与其对应的峰面积呈线性关系，检出限（3S/N）依次为0.020，0.040 mg·L^-1。加标回收率为81.4%~101%，测定值的相对标准偏差（n=6）为1.1%~6.6%。

HPLC was applied to the determination of emission quantity of 2 diisocyanates, i.e. 2,6-TDI and HDI in polyurethane adhesives after application. The sample of polyurethane adhesive was spread on a glass plate, which was then put into the environmental chamber. Glass fiber filter membranes impregnated with 1-2MP were used for air sampling. The filter membranes were removed and put in a bottle. 0.2 mol·L^-1 1-2MP toluene solution, ethyl acetate and acetic anhydride were added in the bottle. Hypersil ODS chromatographic column was used as stationary phase, and a mixture of acetonitrile and acetic acid-sodium acetate buffer solution of pH 6.0 (33+67) was used as mobile phase. Detection at 242 nm was adopted in the determination. Linear relationships between values of peak area and mass concentration of 2,6-TDI and HDI were kept in the same range within 20.0 mg·L^-1, with detection limits(3S/N)of 0.020, 0.040 mg·L^-1, respectively. Values of recovery found by standard addition method were in the range of 81.4%-101%, and values of RSDs (n=6) were in the range of 1.1%-6.6%.