采用高效液相色谱法测定化妆品和洗涤剂中荧光增白剂C.I.85，C.I.210，C.I.220，C.I.351和C.I.353。1.000 g化妆品样品或0.500 g洗涤剂样品加入10.0 mL乙腈（1+2）溶液，涡旋振荡1 min后超声提取10 min，以10 000 r·min^-1转速离心5 min，上清液经0.22 μm滤膜过滤后，在MG C₁₈色谱柱（250 mm×4.6 mm，5 μm）上分离，以乙腈和含2 mmol·L^-1乙酸二正己基铵的10 mmol·L^-1乙酸铵溶液为流动相进行梯度洗脱，采用二极管阵列检测器，在波长350 nm处进行检测。5种荧光增白剂的质量浓度均在5.0~100 mg·L^-1内与对应的色谱峰面积呈线性关系，检出限（3S/N）在4~20 mg·kg^-1之间，测定下限（10S/N）在15~70 mg·kg^-1之间。对空白样品在5.0，50，100 mg·L^-1等3个浓度水平上进行加标回收试验，回收率在80.3%~111%之间。对样品中的5种荧光增白剂进行6次平行测定，测定值的相对标准偏差在2.4%~8.9%之间。

A method of high performance liquid chromatography was developed to determine 5 fluorescent whitening agents, including C.I. 85, C.I. 210, C.I. 220, C.I. 351 and C.I. 353, in cosmetics and detergent. The sample (1.000 g of cosmetics or 0.500 g of detergent) was mixed well with 10.0 mL of acetonitrile (1+2) solution by vortex oscillation for 1 min, and then ultrasonically extracted for 10 min. After the mixture was centrifugated at 10 000 r·min^-1 for 5 min, the supernatant was filtered through a 0.22 μm filter membrane, and seperated on a MG C₁₈ column (250 mm×4.6 mm, 5 μm), with a mixture of acetonitrile and 10 mmol·L^-1 ammonium acetate solution (containing 2 mmol·L^-1 di-n-hexyl-ammonium acetate) for gradient elution. A diode array detector was used for detection at the wavelength of 350 nm. Linear relationships were found between peak areas and mass concentrations of the 5 fluorescent whitening agents in the same range of 5.0-100 mg·L^-1, with detection limits (3S/N) in the range of 4-20 mg·kg^-1, and lower limits of determination (10S/N) in the range of 15-70 mg·kg^-1. Tests for recovery was made to blank samples at mass contentrations of 5.0, 50, 100 mg·L^-1, giving values of recovery between 80.3% and 111%. The 5 fluorescent whitening agents in the samples were determined in parallel for 6 times, and the relative standard deviations of the measured values were between 2.4% and 8.9%.