面膜化妆品样品在酸性条件下水解后，得到的水解产物与1-苯基-3-甲基-5-吡啶啉酮发生衍生化反应，采用高效液相色谱法测定面膜化妆品中透明质酸的含量。以Kromasil C₁₈色谱柱（250 mm×4.6 mm，5 μm）为分离柱，用乙腈和0.025 mol·L^-1乙酸铵溶液以不同比例混合的溶液为流动相进行梯度洗脱，用紫外检测器测定。透明质酸的质量浓度在10.0~1 000 mg·L^-1内与其对应的峰面积呈线性关系，检出限（3S/N）为0.010%。以空白样品为基体进行加标回收试验，所得回收率为101%~106%，回收量的相对标准偏差（n=6）为1.5%~7.2%。

The mask cosmetic sample was hydrolyzed under acidic condition, and then the obtained hydrolysate underwent derivative reaction with 1-phenyl-3-methyl-5-pyridinone. HPLC was applied to the determination of hyaluronic acid in mask cosmetics. Kromasil C₁₈ chromatographic column (250 mm×4.6 mm, 5 μm) was used for separation with mixtures of acetonitrile and 0.025 mol·L^-1 ammonium acetate solution with different mixing ratios as mobile phase in the gradient elution. UV detector was used in the determination. Linear relationship between values of peak area and mass concentration of hyaluronic acid was kept in the range of 10.0-1 000 mg·L^-1, with detection limit (3S/N) of 0.010%. On the base of blank sample, test for recovery was made by standard addition method; values of recovery found were in the range of 101%-106%, with RSDs (n=6) of recovery amounts in the range of 1.5%-7.2%.