取条状橡胶样品（厚约2 mm，宽约1 mm）1.000 g，加入体积比为6：1的乙腈-二氯甲烷混合溶液50 mL，超声提取60 min，所得溶液经0.45 μm滤膜过滤，滤液用于超高效液相色谱法分析。用ZORBAX Eclipse XDB-C₁₈色谱柱（4.6 mm×100 mm，1.8 μm）为固定相，以体积比为40：60的乙腈-pH 4.0的12.5 mmol·L^-1磷酸二氢钠混合溶液为流动相进行等度洗脱，使所测定的6种橡胶助剂得以分离，对6种橡胶助剂在不同波长处进行紫外检测，防老剂6PPD[N-（1，3-二甲基）丁基-N'-苯基对苯二胺]的检测波长为290 nm，防焦剂CTP （N-环己基硫代邻苯二甲酰亚胺）和其他4种硫化促进剂[TBBS （N-叔丁基-2-苯并噻唑次磺酰胺）、MBTS （2，2-二硫化二苯并噻唑）、CBS （N-环己基-2-苯并噻唑次磺酰胺）、DCBS （N，N-二环己基-2-苯并噻唑次磺酰胺）]的检测波长均为226 nm。6种橡胶助剂的标准曲线的线性范围均为5.0~500.0 mg·L^-1，其检出限（3S/N）为0.3~2.5 mg·L^-1。按标准加入法进行回收试验，测得回收率为82.8%~107%，测定值的相对标准偏差（n=5）均小于5.0%。

Rubber sample strips (thickness:ca. 2 mm, width:ca. 1 mm) of 1.000 g were taken and extracted ultrasonically with 50 mL of a mixed solution of CH₃CN and CH₂Cl₂ with the volume ratio of 6:1 for 60 min. The solution was filtered through 0.45 μm filtering membrane and the filtrate was used for UPLC analysis. ZORBAX Eclipse XDB-C₁₈ column (4.6 mm×100 mm, 1.8 μm) was used as stationary phase, and a mixed solution of CH₃CN and 12.5 mmol·L^-1 NaH₂PO₄ (pH 4.0) with the volume ratio of 40:60 was used as mobile phase in isogradient elution, in the course of which the 6 rubber additives[i.e., the anti-aging agent 6PPD (N-(1,3-dimethylbuty)-N'-phenyl-phenylenediamine), the anti-charring agnet CTP (N-cyclohexylthiophthalimide) and 4 vulcanizing agents TBBS (N-tert-butyl-2-benzothiazole), MBTS (2,2-dibenzothiazole disulfide), CBS (N-cyclohexyl-2-benzothiazole sulfenamide) and DCBS (N,N-dicyclohexyl-2-benzothiazole sulfenamide)] were separated and their absorbances measured spectrophotometrically at different wavelengths of 290 nm (for 6PPD) and of 226 nm (for the remainder). Linearity ranges of the standard curves of the 6 rubber additives were found same between 5.0 mg·L^-1 and 500.0 mg·L^-1; their detection limits (3S/N) were found in the range of 0.3-2.5 mg·L^-1. Test for recovery was made by standard addition method, giving recoveries ranged from 82.8% to 107%. Values of RSDs (n=5) were found all less than 5.0%.